HDAC4 Rabbit mAb - P56524

Core Product Specifications and Parameters

Biological Background: HDAC4 Function and Localization

• HDAC4 is a class II histone deacetylase that catalyzes the removal of acetyl groups from lysine residues on the N-terminal tails of core histones (H2A, H2B, H3, H4), contributing to chromatin compaction and epigenetic gene silencing.
• It operates within large multiprotein complexes and plays critical roles in transcriptional regulation, cell cycle progression, and developmental processes.
• HDAC4 is a key regulator of muscle maturation through its interactions with myocyte enhancer factors MEF2A, MEF2C, and MEF2D.
• In breast cancer, it participates in the MTA1-mediated epigenetic regulation of estrogen receptor alpha (ESR1) expression.
• Deacetylation of HSPA1A and HSPA1B at Lys-77 promotes their preferential binding to the co-chaperone STUB1. Related references: PMID:27708256
• The protein localizes to both the nucleus and cytoplasm.
• HDAC4 is ubiquitously expressed and subject to extensive post-translational modifications, including phosphorylation and ubiquitination. Mutations have been linked to neurological disorders such as epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human HDAC4. Verify sequence homology across species and isoforms if cross-reactivity is desired.
• For Western blotting, a band at approximately 119 kDa is expected. Validate the antibody in relevant human cell lines or tissues known to express HDAC4.
• Given the nuclear-cytoplasmic shuttling of HDAC4, subcellular fractionation may enhance detection in specific compartments.
• When using ELISA, consider direct or capture formats and confirm specificity with appropriate controls.

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