HDAC2 Rabbit pAb - Q92769

Core Product Specifications and Parameters

Biological Background: HDAC2 Function and Localization

• HDAC2 (Histone deacetylase 2) catalyzes the deacetylation of lysine residues on core histones (H2A, H2B, H3, H4), leading to epigenetic gene silencing. Related references: PMID:28497810
• It is a core component of transcriptional repressor complexes including SIN3, NuRD, and SIN3B, which mediate chromatin remodeling and gene repression. Related references: PMID:12724404, PMID:16428440, PMID:28977666, PMID:37137925
• HDAC2 belongs to the class I histone deacetylase family and plays essential roles in transcriptional regulation, cell cycle progression, and development.
• The protein is localized in both the nucleus and cytoplasm, enabling deacetylation of nuclear histones and cytoplasmic non-histone substrates.
• HDAC2 deacetylates non-histone proteins such as TSHZ3, TFF1, and CDKN1A, modulating their transcriptional repressor functions. Related references: PMID:19343227, PMID:21965678
• In addition to acetyl group removal, HDAC2 acts as a protein lysine deacylase, removing crotonyl, lactyl, and 2-hydroxyisobutyryl modifications. Related references: PMID:28497810, PMID:29192674, PMID:35044827
• Widely expressed across tissues, with lower levels in brain and lung.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 390-488) of human HDAC2; this region is conserved in mouse and rat, supporting the reported cross-reactivity. Verify sequence alignment for your species of interest.
• For western blotting (WB), a band around 55 kDa is expected. Use nuclear extracts or whole cell lysates from appropriate cell lines (e.g., HeLa, HEK293) and optimize loading and antibody dilution.
• In immunohistochemistry (IHC-P), consider antigen retrieval using citrate buffer and validate with tissues known to express HDAC2 (e.g., liver, kidney). Include isotype controls.
• For immunoprecipitation (IP), this polyclonal antibody may co-immunoprecipitate HDAC2-associated complex components. Optimize lysis conditions to preserve protein interactions.
• ELISA can be performed using the immunizing peptide or recombinant protein as coating antigen; the peptide-based immunogen suggests strong reactivity in direct ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop sourcing solution for IVD raw materials, from early-stage development to clinical application. For epigenetic research, our catalog includes validated antibodies against key chromatin regulators such as HDAC2, alongside recombinant proteins, antigen-grade peptides, and ancillary reagents. With flexible supply scales and dedicated technical support, CamelBio accelerates your assay optimization and commercialization.