hCG Beta Rabbit Monoclonal Antibody for ELISA, WB, IHC-P - P0DN86

Core Product Specifications and Parameters

Biological Background: hCG Beta Function and Localization

• hCG is a heterodimeric glycoprotein hormone composed of a common alpha subunit (shared with LH, FSH, TSH) and a unique beta subunit (CGB3, CGB5, CGB8) that determines biological specificity.
• The beta subunit (hCG Beta) is encoded by the CGB3, CGB5, and CGB8 genes, and undergoes alternative splicing to generate multiple isoforms.
• hCG Beta is essential for pregnancy maintenance, stimulating the corpus luteum to produce progesterone and promoting maternal adaptation.
• The protein is secreted and highly expressed in the placenta throughout pregnancy.
• It is a glycoprotein with multiple disulfide bonds, and its 3D structure has been characterized.
• hCG Beta has pharmaceutical applications, including use in fertility treatments and as a tumor marker.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 21-165 of human hCG Beta1, covering the mature secreted form (signal peptide removed), which likely favors recognition of native and denatured forms.
• For ELISA, consider using this antibody as a capture or detection antibody in sandwich ELISA formats for hCG detection, after validation with relevant standards.
• For Western blot (WB), the predicted molecular weight is approximately 18 kDa; validate the band pattern in relevant samples (e.g., placental lysates, recombinant protein). Note that glycosylation may affect migration.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and validate in placental tissue sections as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this hCG Beta rabbit monoclonal antibody as part of a comprehensive IVD raw-material sourcing platform tailored for reproductive diagnostic development. Beyond premium monoclonal antibodies, we supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets—bridging the gap from concept to clinic with one-stop procurement support.