HCCS Rabbit pAb - P53701

Core Product Specifications and Parameters

Biological Background: HCCS Function and Localization

• Holocytochrome c-type synthase (HCCS), also known as cytochrome c-type heme lyase, catalyzes the covalent attachment of a heme group to apocytochrome c, a critical step for mature cytochrome c production and mitochondrial respiratory chain function.
• Encoded by the HCCS gene (Gene ID: 3052) in humans, mutations are linked to microphthalmia with linear skin defects (MLS) syndrome (also designated MCOPS7) and related mitochondrial disorders, highlighting its essential role in development and energy metabolism.
• HCCS is an integral membrane protein located at the mitochondrial inner membrane, where its active site faces the intermembrane space, directly coupling heme to apocytochrome c.
• The protein undergoes N‑terminal myristoylation and is classified as a lipoprotein; this lipid modification likely facilitates membrane anchoring and influences enzyme stability.
• Alternative names include CCHL (cytochrome c‑type heme lyase), reflecting its enzymatic activity, and MLS, derived from the associated disease phenotype; the protein has a predicted molecular weight of approximately 31 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full‑length human HCCS (residues 1–268), generating a polyclonal antibody that recognizes multiple epitopes; consider peptide‑block experiments to confirm specificity in your sample system.
• For Western blot, a dominant band near 31 kDa is expected in human, mouse, or rat cell lysates; use mitochondrial‑enriched fractions to enhance signal and reduce background.
• In immunofluorescence/ICC, permeabilization conditions should be optimized to detect the mitochondrial inner membrane target; co‑staining with a mitochondrial marker (e.g., Tom20 or MitoTracker) is recommended to confirm localization.
• For ELISA applications, preliminary checkerboard titrations will help establish suitable coating and detection conditions; pair this antibody with a matched detection reagent as part of a customized sandwich immunoassay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined, one‑stop access to IVD raw materials and technical consultation—from concept through clinical readiness. This HCCS antibody can be procured alongside validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary supplies, and rare‑target sourcing support, all tailored for mitochondrial disease research and related diagnostic development.