HCAR3 Rabbit Polyclonal Antibody for WB - P49019

Core Product Specifications and Parameters

Biological Background: HCAR3 Function and Localization

• HCAR3, also known as GPR109B, HM74, or NIACR2, is a G protein-coupled receptor primarily activated by the fatty acid beta-oxidation intermediate 3-hydroxyoctanoate.
• Signals through the inhibitory G(i)/o family of G proteins upon ligand binding, modulating intracellular cAMP levels (PubMed:19561068, PubMed:37736747, PubMed:39427321). Related references: PMID:19561068 | PMID:37736747 | PMID:39427321
• Acts as a key negative feedback regulator of adipocyte lipolysis, counteracting prolipolytic signals during physiological or pathological increases in beta-oxidation (PubMed:19561068). Related references: PMID:19561068
• Functions as a low affinity receptor for nicotinic acid (niacin), responding only at high pharmacological doses beyond normal dietary intake (PubMed:12522134). Related references: PMID:12522134
• Localizes to the cell membrane, consistent with its role as a transmembrane receptor.
• Tissue expression is largely restricted to adipose tissue and spleen, aligning with its metabolic regulatory function.
• Contains characteristic structural features of class A GPCRs, including seven transmembrane helices, a disulfide bond, and potential post-translational modification sites (UniProt keywords: Disulfide bond, Transmembrane, Transmembrane helix).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular region (aa 300-392) of human HCAR3; cross-reactivity with mouse and rat likely stems from sequence conservation in this domain.
• For Western blotting, expect a band near the predicted molecular weight of 44 kDa; validate with appropriate positive controls (e.g., adipose tissue lysates) and consider optimizing antibody concentration.
• When performing IHC-P, use standard paraffin-embedded tissue sections and antigen retrieval methods; spleen or adipose tissue are recommended as positive controls due to enriched HCAR3 expression.
• For ELISA applications, coating with the immunizing peptide or recombinant HCAR3 protein may improve assay performance; titrate antibody and detection reagents for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supplier offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents for diagnostic and research applications. This HCAR3 rabbit polyclonal antibody supports studies in metabolic signaling and GPCR biology, and we can provide it in bulk quantities along with customized sourcing solutions. From validated antibody pairs to rare target raw materials, CamelBio streamlines your procurement process with technical consultation and flexible supply options.