HBG1/HBG2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P69892

Core Product Specifications and Parameters

Biological Background: HBG1/HBG2 Function and Localization

• HBG2 and HBG1 encode the gamma-2 (G-gamma) and gamma-1 (A-gamma) globin chains, respectively, which are nearly identical and together compose the gamma subunits of fetal hemoglobin (HbF).
• Fetal hemoglobin is a tetramer formed by two gamma chains and two alpha chains, and it exhibits a higher oxygen affinity than adult hemoglobin, facilitating oxygen transfer from mother to fetus.
• Both genes belong to the beta-globin gene cluster and are predominantly expressed during fetal development, with expression declining after birth as adult beta-globin synthesis begins.
• Hemoglobin is localized in the cytoplasm of red blood cells, where it performs oxygen transport and delivery.
• Post-translational modifications such as acetylation, phosphorylation, and S-nitrosylation have been reported for gamma-2 globin and may modulate hemoglobin function or stability.
• The gamma-2 chain is classified under keywords including heme, iron, metal-binding, oxygen transport, and transport, reflecting its essential role in oxygen carriage.
• Disease variants in HBG2 and HBG1 are linked to hereditary persistence of fetal hemoglobin (HPFH) and other benign or pathological hemoglobinopathies.
• Tissue specificity is limited to red blood cells, consistent with the primary site of hemoglobin production.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100) of human HBG1/HBG2; due to the high sequence identity between the two gamma chains, this antibody is expected to recognize both HBG1 and HBG2 products.
• For western blotting, a band at approximately 16 kDa should be observed; validate the target identity using appropriate positive and negative controls, as cross-reactivity with other globin chains cannot be excluded.
• For IF/ICC, consider permeabilization conditions to allow antibody access to cytoplasmic hemoglobin; note that red blood cells may require specialized fixation or isolation protocols.
• In ELISA applications, the antibody may be used for quantitative detection of fetal hemoglobin levels; ensure specificity testing against adult hemoglobin if discrimination is required.

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CamelBio serves as a comprehensive partner for IVD developers, laboratories, and researchers by supplying high-quality raw materials such as this anti-HBG1/HBG2 polyclonal antibody for hemoglobin and hematological disorder studies. Our portfolio extends to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials, all supported by technical and consulting services. Contact us to streamline your development workflow from concept to clinical application.