HBG1/2 Rabbit mAb (WB, IHC, IF, ELISA) - P69891/P69892

Core Product Specifications and Parameters

Biological Background: HBG1/2 Function and Localization

• Hemoglobin subunit gamma-1 (HBG1) and gamma-2 (HBG2) are the gamma-globin chains that, together with alpha-globin, form fetal hemoglobin (HbF, α2γ2), the predominant hemoglobin during fetal development.
• HbF has a higher oxygen affinity than adult hemoglobin (HbA), facilitating efficient oxygen transfer across the placenta from mother to fetus.
• Expression of gamma-globin genes is primarily restricted to red blood cells and is developmentally regulated, with a switch from gamma- to beta-globin expression occurring around birth.
• Postnatally, residual HbF is present at low levels in adults, and its reactivation is a therapeutic goal for sickle cell disease and beta-thalassemia.
• HBG1 and HBG2 are highly homologous and located within the human beta-globin gene cluster on chromosome 11p15.4.
• The protein undergoes various post-translational modifications, including acetylation, phosphorylation, and S-nitrosylation, which may influence its stability and function.
• Keywords related to HBG1 include 3D-structure, heme, iron, metal-binding, oxygen transport, and reference proteome, highlighting its central role in oxygen delivery.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the N-terminal region (aa 1-100) of HBG1, which may detect both HBG1 and HBG2 due to high sequence similarity.
• The observed protein weight in SDS-PAGE is approximately 12 kDa, consistent with the monomeric form of gamma-globin. Validate band size using appropriate molecular weight markers and fetal hemoglobin-positive control lysates.
• For Western blotting, consider using red blood cell lysates from fetal or cord blood samples, or cell lines induced to express fetal hemoglobin.
• In IHC and IF applications, expect cytoplasmic staining in erythroid cells. Paraffin-embedded and frozen sections of fetal liver or bone marrow are suitable samples.
• ELISA performance can be assessed using recombinant or purified fetal hemoglobin protein. Pairing with an alpha-globin antibody may enable sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, core labs, and research institutes with streamlined access to premium IVD raw materials, covering every stage from concept to clinic. This HBG1/2 rabbit monoclonal antibody is part of our portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to support hematology and developmental biology research and assay development. Count on CamelBio as your strategic partner for reliable, high-quality raw materials that accelerate your diagnostic pipeline.