HAUS8 Rabbit pAb for WB - Q9BT25

Core Product Specifications and Parameters

Biological Background: HAUS8 Function and Localization

• HAUS8 (HAUS augmin-like complex subunit 8) is also known as HEC1/NDC80-interacting centrosome-associated protein 1 (HICE1) and sarcoma antigen NY-SAR-48.
• It is a component of the HAUS augmin-like complex, which plays a critical role in mitotic spindle assembly by generating microtubules and maintaining centrosome integrity.
• The HAUS complex contributes to proper chromosome segregation by ensuring timely completion of cytokinesis.
• HAUS8 localizes to the cytoplasm, and during mitosis, it concentrates at the centrosome, mitotic spindle, and spindle poles.
• The protein is subject to post-translational modifications, including acetylation and phosphorylation, which may regulate its activity during the cell cycle.
• Alternative splicing of HAUS8 results in multiple isoforms, potentially expanding functional diversity.
• Keywords associated with HAUS8 include cell cycle, cell division, mitosis, microtubule, and coiled coil, reflecting its structural and regulatory features.

Experimental Guidance and Technical Tips

• For WB, the predicted molecular weight is 45 kDa (UniProt 44.9 kDa); consider including appropriate molecular weight markers and validate with positive and negative controls.
• This antibody, raised against a recombinant protein spanning amino acids 166–410, is suitable for detecting endogenous HAUS8 across human and mouse samples; consider optimizing dilution for each application.
• For IF/ICC, fixation and permeabilization conditions should be optimized to preserve spindle and centrosome structures; co-staining with centrosome markers (e.g., γ-tubulin) may aid specificity.
• Since HAUS8 is expressed throughout the cell cycle, synchronization protocols may enhance detection in specific mitotic phases.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive portfolio of high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this HAUS8-targeting antibody, to support your cell cycle and mitosis research. Our one-stop sourcing covers bulk orders, ancillary reagents, and custom sourcing for rare targets, streamlining your development from concept to clinic.