HARS2 Rabbit pAb for WB, ELISA - P49590

Core Product Specifications and Parameters

Biological Background: HARS2 (Mitochondrial Histidine--tRNA Ligase) Function and Localization

• HARS2 encodes mitochondrial histidine--tRNA ligase, also known as histidyl-tRNA synthetase (HisRS) and histidine--tRNA ligase-like. Alternative names include HARSL, HARSR, HO3, and PRLTS2.
• This enzyme belongs to the class II aminoacyl-tRNA synthetase family and catalyzes the ATP-dependent attachment of histidine to its cognate tRNA(His), forming histidyl-tRNA(His) and AMP.
• HARS2 is localized to the mitochondrion, where it plays a critical role in mitochondrial protein translation by providing charged tRNA(His) for ribosome-mediated polypeptide elongation.
• High expression is observed in heart, kidney, and skeletal muscle, with lower levels in brain and liver, reflecting tissue-specific mitochondrial activity.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity or stability (Keywords: Acetylation, Phosphoprotein).
• Mutations in HARS2 are associated with Perrault syndrome 2 (PRLTS2), a disorder involving sensorineural hearing loss and ovarian dysfunction, underscoring its clinical significance in mitochondrial disease.

Experimental Guidance and Technical Tips

• For WB, a band around 57 kDa is expected, but note that endogenous cleavage or post-translational modifications may result in additional bands. Validate in relevant sample systems.
• As a polyclonal antibody, it is well-suited for ELISA due to its ability to bind multiple epitopes; consider optimizing coating and detection conditions.
• The immunogen corresponds to a region conserved among human, mouse, and rat, supporting cross-reactivity. Validate specificity in samples from these species to confirm.
• Since HARS2 is mitochondrial, ensure lysis buffers are compatible with mitochondrial protein extraction if targeting endogenous protein in tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This HARS2 antibody supports research into mitochondrial translation and related diseases. Contact us for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your project.