GUCY2C Rabbit Polyclonal Antibody for WB and ELISA - P25092

Core Product Specifications and Parameters

Biological Background: Guanylyl cyclase C Function and Localization

• GUCY2C encodes guanylyl cyclase C, a transmembrane receptor that catalyzes the conversion of GTP to cyclic GMP (cGMP), acting as a key regulator of intestinal fluid homeostasis and cell proliferation.
  Related references: PMID:11950846, PMID:1718270, PMID:22436048

• It functions as the receptor for heat-stable enterotoxins produced by E. coli, which potently stimulate cGMP accumulation in mammalian cells.
  Related references: PMID:1680854, PMID:1718270

• The receptor is also activated by the endogenous peptides guanylin and uroguanylin, linking it to physiological regulation of ion transport and epithelial homeostasis.
  Related references: PMID:8381596

• Subcellular localization includes the cell membrane and endoplasmic reticulum membrane, consistent with its role as a transmembrane receptor.

• Predominantly expressed in the intestinal brush border, where it mediates enterotoxin-induced diarrhea and physiological guanylin/uroguanylin signaling.

• The protein is a glycosylated transmembrane receptor with lyase and GTP-binding activity, and has known 3D structures and disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 24-330), likening it to detect both full-length and potential shed extracellular fragments in Western blot.
• For WB, use a positive control lysate from human colorectal cell lines (e.g., CaCo-2, T84) or mouse intestinal tissue to confirm specific detection of the ~123 kDa band.
• In ELISA, recombinant GUCY2C extracellular domain protein can serve as a coating standard; validate linearity and limit of detection under your assay conditions.
• Optimize antibody dilution empirically; typical polyclonal antibodies may require a range of 1:500-1:5000 for WB and 1:1000-1:10000 for direct ELISA, depending on detection method and sample abundance.
• Note cross-reactivity with mouse GUCY2C; confirm specificity in your target species using knockout controls or peptide competition if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting — covering every stage from concept to clinic. For gastrointestinal disease and enterotoxin research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-GUCY2C pAb, bulk ancillary reagents, and rare target raw-material sourcing. Our integrated platform streamlines your development workflow, from initial reagent selection to scalable production.