GTF2I Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P78347

Core Product Specifications and Parameters

Biological Background: GTF2I Function and Localization

• GTF2I, also known as TFII-I, BAP-135, and WBSCR6, is a general transcription factor that acts as a signal-responsive regulator linking specific activators to the basal transcription machinery.
• It promotes formation of stable SRF-PHOX1 complexes and cooperatively enhances serum-inducible c-FOS transcription through the SRE, and also functions as a coregulator for USF1 at initiator and E-box elements.
• GTF2I is required for functional ARID3A DNA-binding complexes and activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation, highlighting its role in immune cell signaling.
• The protein localizes to both the cytoplasm and nucleus, and is subject to multiple PTMs including acetylation, phosphorylation, and ubiquitin-like conjugation, which may modulate its activity.
• Tissue expression is ubiquitous, with isoform 1 showing high expression in fetal brain but low in adult tissues, while other isoforms are more uniformly expressed across adult tissues.
• GTF2I is implicated in Williams-Beuren syndrome, a neurodevelopmental disorder characterized by cardiovascular and cognitive features, consistent with its chromosomal location in the WBS critical region.
• It contains several repeat domains and functions via DNA-binding at pyrimidine-rich initiator (Inr) elements and E-boxes, contributing to diverse transcriptional programs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 600-810 of human GTF2I, a region that may confer specific reactivity; researchers should validate antibody specificity in their target species and tissue context.
• For western blotting (WB), the predicted molecular weight is approximately 112 kDa; consider using appropriate protein ladders and loading controls for accurate size determination.
• In immunohistochemistry (IHC-P), GTF2I localizes to both cytoplasm and nucleus, so positive staining may be observed in both compartments; optimize antigen retrieval and blocking conditions.
• For ELISA, the antibody may be suitable for capturing or detecting GTF2I; perform a preliminary checkerboard titration to establish optimal working concentrations.
• Due to the polyclonal nature, batch-to-batch consistency should be verified when switching lots; consider running a side-by-side comparison with previous lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For GTF2I, a key transcription factor associated with Williams-Beuren syndrome and immunoglobulin gene regulation, we offer tailored solutions to support your diagnostic and research applications. Contact us to explore our full portfolio and custom procurement services.