GTF2F1 Rabbit Polyclonal Antibody for WB, ELISA - P35269

Core Product Specifications and Parameters

Biological Background: GTF2F1 Function and Localization

• GTF2F1 encodes the 74 kDa alpha subunit of the general transcription factor IIF (TFIIF), also known as RAP74 or TFIIF-alpha.
• TFIIF serves as a general transcription initiation factor that binds to RNA polymerase II and facilitates its recruitment to the preinitiation complex in cooperation with TFIIB.
• The factor promotes transcription elongation, supporting the transition from initiation to productive RNA synthesis.
• The protein is localized in the nucleus, consistent with its role in transcription.
• Post-translational modifications include acetylation and phosphorylation, as indicated by UniProt keywords.
• Additional keywords from UniProt include DNA-binding, transcription, and transcription regulation, reflecting its central role in gene expression.
• The UniProt reviewed entry notes a molecular weight of 58,240 Da, in agreement with the observed 58 kDa band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 390-517, a region within the C-terminal portion of GTF2F1; target the same region in your experiments to ensure recognition.
• As a rabbit polyclonal antibody, it offers robust signal amplification but may exhibit batch-to-batch variability; validate each lot for critical applications.
• For Western blotting, a starting dilution of 1:500-1:2000 is commonly used with whole-cell lysates; optimize based on target abundance and sample system.
• In ELISA, consider coating the antigen directly or using a sandwich format; test serial dilutions to establish optimal antibody concentration.
• Cross-reactivity has been demonstrated with human, mouse, and rat; verify reactivity in your specific tissue or cell line before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing bridge, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This GTF2F1 antibody supports transcription research and assay development projects. For bulk orders or technical inquiries, our team is ready to assist.