GSTA4 Rabbit mAb for WB, ELISA - O15217

Core Product Specifications and Parameters

Biological Background: GSTA4 Function and Localization

• GSTA4, also known as glutathione S-transferase A4-4, is a member of the alpha class of glutathione S-transferases, a family of detoxification enzymes.
• Its primary function is to catalyze the conjugation of reduced glutathione to a wide range of exogenous and endogenous hydrophobic electrophiles, playing a critical role in cellular defense against oxidative stress.
• Notably, GSTA4 exhibits high catalytic efficiency toward 4-hydroxyalkenals, particularly 4-hydroxynonenal (4-HNE), a major product of lipid peroxidation, implicating it in the regulation of lipid peroxidation-related signaling pathways.
• The enzyme is predominantly localized in the cytoplasm, consistent with its role in detoxifying reactive molecules generated within the cytosol.
• Tissue expression analysis reveals high levels in brain, placenta, and skeletal muscle, with significantly lower expression in lung and liver, suggesting tissue-specific detoxification requirements.
• Post-translational modifications including acetylation have been identified, and alternative splicing generates multiple transcript variants (UniProt evidence at protein level).

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 1-100) spans the N-terminal region of GSTA4, which is conserved among alpha class GSTs; consider potential cross-reactivity with other GST alpha family members when high specificity is required.
• This antibody is validated for Western blot (WB) and ELISA; for WB, expect a band around 26 kDa. Optimize loading and blocking conditions based on sample lysate.
• When using ELISA, ensure coating with the recombinant or native protein at an appropriate concentration and validate in the relevant sample matrix.
• Given the tissue distribution, consider using brain, placenta, or skeletal muscle lysates as positive controls, while lung or liver samples may show weak or no signal.
• For applications beyond WB and ELISA, perform your own validation as the antibody's performance in other assays (e.g., IHC, IP) has not been specified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibodies like this GSTA4 rabbit monoclonal. Whether you need optimized antibody pairs for glutathione S-transferase assays or bulk ancillary reagents for oxidative stress panels, our one-stop supply chain supports your workflow from concept to clinic. Contact us to explore custom sourcing for rare targets in detoxification and lipid peroxidation pathways.