GSTA2 Rabbit Polyclonal Antibody for WB, ELISA - P09210

Core Product Specifications and Parameters

Biological Background: GSTA2 Function and Localization

• GSTA2, also known as glutathione S-transferase A2, GST HA subunit 2, GST class-alpha member 2, or GST-gamma, is a member of the alpha class glutathione S-transferase family (Gene: GSTA2).
• It catalyzes the conjugation of reduced glutathione to a wide variety of electrophilic compounds, playing a central role in cellular detoxification and xenobiotic metabolism.
• GSTA2 is predominantly expressed in the liver, highlighting its importance in hepatic detoxification pathways.
• The enzyme localizes to the cytoplasm, where it facilitates the interaction between glutathione and hydrophobic substrates.
• Post-translational modifications include acetylation, as identified through proteomic approaches.
• Its three-dimensional structure has been resolved, contributing to understanding of substrate specificity and catalytic mechanism.
• The protein has been characterized by direct protein sequencing and extensive proteomics identification, confirming its expression and modifications.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein encompassing amino acids 1-222 of human GSTA2 (full-length), which may offer broad epitope coverage and robust signal generation.
• This polyclonal antibody is suitable for Western blot (WB) and ELISA. For WB, a band of approximately 26 kDa is expected; validation in relevant sample systems (e.g., liver lysates) is recommended.
• Cross-reactivity with mouse and rat GSTA2 suggests potential application in rodent models; verify reactivity in your specific experimental conditions.
• For ELISA, pair this antibody with an appropriate detection antibody and optimize coating and blocking conditions for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing for detoxification enzyme and liver biology research. In addition to this GSTA2 antibody, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets—supporting your diagnostic development from concept to clinic.