GSK3β Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P49841

Core Product Specifications and Parameters

Biological Background: GSK3β Function and Localization

• Glycogen synthase kinase-3 beta (GSK3β), also known as serine/threonine-protein kinase GSK3B, is encoded by the GSK3B gene and acts as a constitutively active kinase that regulates numerous signaling pathways, including hormonal control of glucose homeostasis and Wnt signaling (PubMed:11430833, PubMed:12554650, PubMed:14690523). Related references: PMID:11430833, PMID:12554650, PMID:14690523

• In glucose metabolism, GSK3β phosphorylates and inactivates glycogen synthase (GYS1), contributing to insulin-regulated glycogen synthesis in skeletal muscle, and may also mediate insulin resistance via transcription factor regulation (PubMed:8397507). Related references: PMID:8397507

• As a central component of the Wnt pathway, GSK3β forms a complex with APC, AXIN1, and β-catenin (CTNNB1), phosphorylating β-catenin’s N-terminus to promote its ubiquitin/proteasome-mediated degradation (PubMed:12554650). Related references: PMID:12554650

• GSK3β phosphorylates MAPT/TAU at ‘Thr-548’, reducing its microtubule-binding ability and contributing to neurofibrillary tangle formation in Alzheimer disease (PubMed:14690523). Related references: PMID:14690523

• Beyond metabolism and Wnt, it modulates transcription factors (e.g., JUN, NFATC1), regulates circadian clock components (BMAL1, CLOCK, PER2), and influences neuronal polarity, autophagy, and apoptosis via diverse substrate phosphorylation.

• The protein is located in the cytoplasm, nucleus, and cell membrane, and shows tissue-specific expression with highest levels in testis, thymus, prostate, and ovary, weaker in lung, brain, and kidney, with notable colocalization in Alzheimer’s disease brain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 350–420), an epitope region conserved across human, mouse, and rat, supporting multi-species detection.
• For Western blot (WB), a band at approximately 47 kDa is expected; consider using appropriate loading controls and verifying band identity with positive tissue or cell lysates.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), evaluate antibody dilution and antigen retrieval conditions to optimize specific staining, particularly for nuclear or cytoplasmic targets.
• The antibody is applicable to immunoprecipitation (IP) and ELISA; validate performance in your sample system with recommended controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-GSK3β reagent, and bulk ancillary reagents. Focused on key signaling and disease areas such as metabolism, neurology, and oncology, CamelBio’s extensive portfolio supports everything from biomarker discovery to clinical assay development. Connecting you to rare-target sourcing and technical consultation, CamelBio bridges the gap from concept to clinic.