Grp75/MOT/HSPA9 Rabbit mAb for WB, ELISA - P38646

Core Product Specifications and Parameters

Biological Background: Grp75/MOT/HSPA9 Function and Localization

• Encoded by the HSPA9 gene, this protein is a mitochondrial member of the heat shock protein 70 (HSP70) family, also known as mortalin or GRP-75.
• Functions as a key mitochondrial chaperone, facilitating protein import through association with the TIM complex and ensuring proper folding and assembly of mitochondrial proteins via ATP-dependent cycles. Related references: PMID:18632665, PMID:25615450, PMID:28848044
• Essential for mitochondrial iron-sulfur cluster (ISC) biogenesis by stabilizing assembly proteins FXN, NFU1, NFS1, and ISCU, and regulates erythropoiesis through ISC stabilization. Related references: PMID:21123823, PMID:26702583
• Mediates mitochondrial calcium-dependent apoptosis by coupling ITPR1 and VDAC1 at the ER-mitochondria interface, facilitating calcium transfer to the mitochondrial matrix.
• In the cytosol, interacts with and promotes degradation of TP53, potentially influencing cell cycle regulation and senescence. Related references: PMID:24625977, PMID:26634371
• Localizes to multiple cellular compartments including the mitochondrial matrix, nucleus, nucleolus, and cytoplasm, reflecting its diverse functional roles.
• Exhibits extracellular cytoprotective activity by disrupting formation of the membrane attack complex, preventing cell lysis following immune attack. Related references: PMID:16091382
• Contains post-translational modifications such as acetylation, methylation, and phosphorylation, and bears an N-terminal transit peptide for mitochondrial targeting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region of human HSPA9 (aa 580–679), which is likely conserved across species, consistent with the reported cross-reactivity with mouse and rat.
• For Western blotting, consider using reducing conditions and validate the detected band against the predicted molecular weight of ~74 kDa.
• ELISA applications may require optimization of coating antigen and antibody concentration; using recombinant protein or peptide as a standard is recommended.
• This monoclonal antibody offers consistent batch-to-batch reproducibility, suitable for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-Grp75/MOT/HSPA9 rabbit mAb for mitochondrial biology and stress response studies. We supply bulk antibody production, optimized antibody pairs, and ancillary reagents, backed by technical consulting to accelerate your assay development from concept to clinic.