GRIK2 Rabbit mAb for WB, IHC-P, ELISA - Q13002

Core Product Specifications and Parameters

Biological Background: GRIK2 Function and Localization

• GRIK2, also known as glutamate receptor 6 (GluR6) or excitatory amino acid receptor 4 (EAA4), is a member of the ionotropic glutamate receptor family that functions as a cation-permeable ligand-gated ion channel gated by L-glutamate and the agonist kainate, mediating fast excitatory synaptic transmission in the central nervous system (PubMed:7536611, PubMed:8730589). Related references: PMID:7536611 PMID:8730589
• Upon agonist binding, GRIK2 undergoes rapid desensitization, entering a transient inactive state that shapes synaptic responses and neuronal excitability (PubMed:14511640, PubMed:28180184, PubMed:34375587). Related references: PMID:14511640 PMID:28180184 PMID:34375587
• In addition to its ionotropic role, GRIK2 functions as a thermoreceptor conferring sensitivity to cold temperatures, with expression observed in dorsal root ganglion neurons (PubMed:31474366). Related references: PMID:31474366
• GRIK2 is localized to the cell membrane, predominantly at postsynaptic sites, where it contributes to postsynaptic excitation.
• The receptor can form heteromers with GRIK3 to modulate presynaptic glutamate release at hippocampal mossy fiber synapses, influencing synaptic plasticity.
• Expression of GRIK2 is notably higher in the cerebellum than in the cerebral cortex, suggesting region-specific roles in motor control and coordination.
• GRIK2 undergoes alternative splicing and RNA editing, generating multiple isoforms that diversify its functional and regulatory properties. It is annotated with keywords including epilepsy, intellectual disability, and disease variant, reflecting its clinical relevance.

Experimental Guidance and Technical Tips

• For Western blotting, GRIK2 protein has a predicted molecular weight of ~103 kDa. Ensure adequate separation on SDS-PAGE gels and validate with a suitable loading control.
• For immunohistochemistry (paraffin), antigen retrieval may be required due to the transmembrane nature of GRIK2. Consider citrate buffer (pH 6.0) or Tris-EDTA (pH 9.0) protocols and validate in appropriate tissue samples.
• ELISA can be used for quantitative detection of GRIK2 in tissue lysates. Include a synthetic peptide corresponding to the immunogen as a positive control to verify assay specificity.

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