GRHL2 Rabbit Polyclonal Antibody for WB, ELISA - Q6ISB3

Core Product Specifications and Parameters

Biological Background: GRHL2 Function and Localization

• GRHL2 (Grainyhead-like protein 2 homolog), also known as Brother of mammalian grainyhead (BOM) and Transcription factor CP2-like 3 (TFCP2L3), is a member of the grainyhead-like transcription factor family that includes GRHL1, GRHL2, and GRHL3.
• Plays a critical role in primary neurulation and epithelial development by acting as both a transcriptional activator and repressor, binding to the consensus DNA sequence 5'-AACCGGTT-3'. Related references: PMID:25152456, PMID:29309642
• Cooperates with GRHL3 to establish distinct zones of primary neurulation; essential for closure 3 (rostral forebrain) and posterior neuropore closure.
• Regulates epithelial morphogenesis by up-regulating tight junction components CLDN3 and CLDN4, and enhances CLDN4 localization at tight junctions via RAB25.
• In keratinocytes, activates telomerase by binding to the TERT promoter and inhibiting DNA methylation, likely by interfering with DNMT1 activity. Related references: PMID:19015635, PMID:20938050
• Suppresses keratinocyte differentiation and epidermal function by epigenetically inhibiting expression of epidermal differentiation complex (EDC) genes, as well as GRHL1 and GRHL3. Related references: PMID:23254293
• Localizes to the nucleus and membrane. Highly expressed in keratinocytes, placenta, prostate, brain, kidney, and various epithelial tissues including lung and cochlea; also detected in corneal epithelial cells. Related references: PMID:29499165
• The gene undergoes alternative splicing, producing protein isoforms with calculated molecular weights of approximately 69 kDa and 71 kDa, consistent with observed bands in Western blot. Mutations in GRHL2 are associated with diseases such as corneal dystrophy, deafness, and ectodermal dysplasia (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 110–210 of human GRHL2, a region that may be conserved across species; given reported cross-reactivity with human and rat, validation in other species (e.g., mouse) should be performed if required.
• For Western blotting, use whole-cell lysates from GRHL2-expressing cells such as HaCaT (human keratinocytes), placental tissue, or prostate cancer cell lines. The antibody is expected to detect bands at approximately 69 kDa and 71 kDa, corresponding to the two major isoforms.
• For ELISA, consider the application’s requirement for native versus denatured protein recognition, and include appropriate controls.
• Since the antibody is a polyclonal rabbit IgG, standard anti-rabbit secondary antibodies are suitable; optimize primary antibody dilution for each assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This GRHL2 rabbit polyclonal antibody, targeting a key transcription factor in epithelial development and neurulation, is part of our extensive portfolio. We also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare target materials to streamline your assay development.