GRB2 Rabbit Monoclonal Antibody for WB - P62993

Core Product Specifications and Parameters

Biological Background: GRB2 Function and Localization

• GRB2 is a non-enzymatic adapter protein that serves as a critical link in signaling cascades from cell surface receptors to downstream cellular responses, including gene expression and various biological processes such as innate and adaptive immunity, autophagy, DNA repair, and necroptosis. Related references: PMID:11016927, PMID:11726515, PMID:37626338
• In T-cells, GRB2 binds to phosphorylated LAT upon TCR engagement, recruiting SOS1 to activate the KRAS/RAF1/MAPK pathway, thereby regulating T-cell activation, differentiation, and function. Related references: PMID:9489702, PMID:12171928, PMID:25870599, PMID:36864087
• Similarly, in B-cells, GRB2 amplifies Ca2+ mobilization and ERK MAP kinase activation upon recruitment to the phosphorylated BCR. Related references: PMID:25413232, PMID:29523808
• GRB2 regulates the switch between autophagy and programmed necrosis by interacting with necrosome components (e.g., RIPK1) and autophagy regulators (e.g., SQSTM1, BECN1). Related references: PMID:35831301, PMID:38182563
• It modulates miRNA biogenesis through formation of a ternary complex with AGO2 and DICER1. Related references: PMID:37328606
• GRB2 participates in the replication stress response and homology-directed repair (HDR) by stabilizing RAD51 at stalled forks and regulating MRE11 recruitment. Related references: PMID:34348893, PMID:38459011
• A naturally occurring isoform (Grb3-3) lacks SH2 domain function and acts as a dominant negative, inhibiting RAS-ERK signaling and cell proliferation by competing with GRB2 for SOS1 binding. Related references: PMID:36171279
• GRB2 is primarily localized in the cytoplasm but also found in the nucleus, endosomes, and Golgi apparatus, consistent with its roles in signal trafficking.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination (isopeptide bond), and it harbors SH2 and SH3 domains that mediate interactions with phosphotyrosine-containing proteins and proline-rich motifs.

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal SH3 domain and adjacent region, which may recognize full-length GRB2 and its isoforms; verify reactivity in your model system.
• For Western blot, GRB2 typically migrates at ~25 kDa; use appropriate loading controls and validate band specificity in knockdown or knockout samples.
• In immunoprecipitation, consider using a crosslinking protocol or a light-chain-specific secondary antibody to avoid interference from the ~25 kDa IgG light chain.
• For immunohistochemistry and immunofluorescence, optimization of antigen retrieval and blocking steps is recommended for each tissue type.
• The antibody's cross-reactivity with mouse and rat GRB2 permits cross-species studies, but confirm with species-specific positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and life science researchers a seamless pathway to secure high-quality IVD raw materials and technical expertise. This GRB2 monoclonal antibody supports investigations into signal transduction, immunology, and cancer biology, backed by our commitment to batch-to-batch consistency. Complement your assay development with our range of recombinant proteins, universal blocking reagents, and custom antibody services, and let us accelerate your path from target identification to clinical application.