GRB10 Rabbit Polyclonal Antibody for WB, ELISA - Q13322

Core Product Specifications and Parameters

Biological Background: GRB10 Function and Localization

• GRB10 (Growth factor receptor-bound protein 10), also known as Grb10 adapter protein or insulin receptor-binding protein Grb-IR, is encoded by the GRB10 gene (human GeneID: 2887) and belongs to the GRB7/10/14 family of adapter proteins.
• Acts as an adapter protein modulating coupling of activated receptor tyrosine kinases with downstream signaling pathways; specifically binds to and suppresses signals from insulin (INSR) and insulin-like growth factor (IGF1R) receptors via two mechanisms: interference with signaling and promotion of receptor degradation.
• Negatively regulates insulin signaling by delaying and reducing AKT1 phosphorylation, blocking INSR association with IRS1/IRS2, and preventing insulin-stimulated IRS1/IRS2 tyrosine phosphorylation.
• Mediates ubiquitination-dependent receptor downregulation by recruiting NEDD4 to IGF1R, leading to increased internalization and degradation via proteasomal and lysosomal pathways; may also promote INSR ubiquitination and proteasomal degradation.
• Negatively regulates Wnt signaling by interacting with the intracellular portion of LRP6 and interfering with AXIN1 binding to LRP6.
• Positively regulates the KDR/VEGFR-2 signaling pathway, possibly by inhibiting NEDD4-mediated degradation of KDR.
• Expressed widely in fetal and adult tissues, including liver, lung, kidney, skeletal muscle, heart, spleen, skin, and brain; subcellular localization is primarily cytoplasmic.
• Features a C-terminal SH2 domain and is subject to phosphorylation and alternative splicing (UniProt Keywords: Alternative splicing, Phosphoprotein, SH2 domain).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human GRB10. For WB, consider using a reduced denaturing gel and validate with positive controls from human cell lines known to express GRB10 (e.g., HepG2, HEK293).
• As a polyclonal antibody, it may recognize multiple epitopes, potentially detecting isoforms or degradation products. Confirm specificity by peptide blocking or knockout validation.
• For ELISA, coat with the immunogen peptide or recombinant GRB10 protein; perform standard indirect ELISA and include appropriate negative controls.
• Cross-reactivity is listed as human only; if testing other species, validate reactivity experimentally in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a single access point for IVD raw materials, technical services, and consulting across every development stage. This GRB10 antibody supports research in metabolic disorders, insulin signaling, and cancer, and can be complemented by CamelBio’s offerings of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Partner with us to streamline your supply chain from concept to clinical application.