Granulin Rabbit PolymAb® - P28799

Core Product Specifications and Parameters

Biological Background: Granulin Function and Localization

• Progranulin (PGRN), also known as acrogranin, epithelin precursor, GP88, and PCDGF, is a secreted glycoprotein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing, and cell proliferation.
  Related references: PMID:12526812 PMID:18378771 PMID:28073925
• PGRN regulates protein trafficking to lysosomes and lysosomal enzyme activity, and facilitates lysosomal acidification leading to CTSD degradation.
  Related references: PMID:28453791 PMID:28541286 PMID:28073925
• As a wound-related growth factor, it promotes dermal fibroblast and endothelial cell division, migration, and capillary-like tube formation, and enhances epithelial cell proliferation by blocking TNF-mediated neutrophil activation.
  Related references: PMID:12526812
• In neurons, progranulin modulates inflammation and preserves neuronal survival, axonal outgrowth, and integrity.
  Related references: PMID:18378771
• Subcellularly, progranulin is secreted and also localizes to lysosomes.
• It is highly expressed in kidney and present in inflammatory cells, bone marrow, and epithelial cell lines; additional functions include promoting A431 cell proliferation, inhibiting epithelial cell proliferation and inducing IL-8 secretion, and stabilizing CTSD via direct interaction.

Experimental Guidance and Technical Tips

• The immunogen maps to amino acids 494–593 in the C-terminal region of human progranulin; verify sequence alignment with mouse to confirm expected cross-reactivity.
• For western blotting, full-length progranulin migrates near 64 kDa, but proteolytic processing yields smaller granulin peptides; include appropriate controls and validate in the relevant sample system.
• In IF/ICC, permeabilization steps may be necessary to visualize lysosomal pools; consider co-staining with organelle markers.
• As a secreted protein, progranulin may be enriched in serum-free conditioned medium or after stimulation; optimize sample preparation for ELISA assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing for research and diagnostic development. This granulin antibody supports investigations into lysosomal dysfunction, inflammation, and cell proliferation pathways. For your project needs, CamelBio offers supply chain reliability and technical support, helping you advance from concept to clinic.