GPS1 Rabbit pAb - Q13098

Core Product Specifications and Parameters

Biological Background: GPS1 Function and Localization

• GPS1, also known as COPS1, CSN1, or COP9 signalosome complex subunit 1, is encoded by the GPS1 gene and is a core component of the COP9 signalosome (CSN) complex.
• The CSN complex plays a critical role in regulating the ubiquitin conjugation pathway by mediating the deneddylation of cullin subunits of SCF-type E3 ubiquitin ligase complexes, thereby reducing their ubiquitin ligase activity.
• GPS1 involvement in CSN-dependent phosphorylation of proteins such as p53/TP53, c-jun/JUN, and others, potentially via associated kinases CK2 and PKD, modulates protein stability and degradation.
• The protein suppresses G protein- and mitogen-activated protein kinase-mediated signal transduction, highlighting its regulatory role in cellular signaling.
• GPS1 is localized both in the cytoplasm and nucleus, consistent with its multifaceted functions.
• It is widely expressed across tissues and is subject to alternative splicing and phosphorylation, as indicated by proteomic data.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 258-527) of human GPS1, ensuring recognition of full-length endogenous GPS1 across multiple species.
• For Western blotting, a predominant band around 56 kDa is expected; consider using adequate controls and optimizing lysate preparation to preserve the target protein.
• In immunohistochemistry and immunofluorescence, validate the antibody in the relevant tissue or cell system, as GPS1 is widely expressed in both cytoplasmic and nuclear compartments.
• The antibody is suitable for ELISA; consider direct and sandwich formats based on the specific assay requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with validated IVD raw materials, including GPS1-targeting antibodies for ubiquitin-proteasome and signalosome studies. As a dedicated B2B partner, we offer one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—supporting your workflow from concept to clinical application.