GPRIN1 Rabbit Polyclonal Antibody for WB and ELISA - Q7Z2K8

Core Product Specifications and Parameters

Biological Background: GPRIN1 Function and Localization

• GPRIN1, also known as GRIN1, is a G protein-regulated inducer of neurite outgrowth 1.
• It is suggested to play a role in neurite outgrowth, a critical process in neuronal development.
• GPRIN1 localizes to the cell membrane and is enriched in growth cones, the dynamic tips of elongating neurites.
• The protein is subject to alternative splicing, generating multiple isoforms, and undergoes post-translational modifications including palmitoylation and phosphorylation.
• GPRIN1 is widely expressed in the central nervous system, with the highest expression levels detected in the spinal cord.
• Its predicted molecular weight is approximately 102 kDa, consistent with the observed 102 kDa band in assays.
• Additional keywords such as "Lipoprotein" and "Phosphoprotein" indicate lipid modification and phosphorylation, which may regulate its membrane association and signaling functions.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1-300) of human GPRIN1, which may detect multiple isoforms due to alternative splicing in this region. Consider sequence alignment to assess isoform coverage.
• For Western blot, the predicted molecular weight is ~102 kDa; however, post-translational modifications may cause band shifts. Use appropriate reducing conditions and validate in neuronal cell lines or tissues.
• ELISA performance may be optimized using the recombinant immunogen as a standard. Specificity should be confirmed with control lysates from GPRIN1-expressing and silenced cells.
• Cross-reactivity with mouse and rat has been reported; confirm with species-specific tissue samples to ensure consistent detection.
• As a polyclonal antibody, lot-to-lot variability is possible; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop platform providing IVD raw materials, technical services, and consulting. This anti-GPRIN1 antibody supports neuroscience research into neurite outgrowth and signaling. From validated antibody pairs to bulk ancillary reagents and custom sourcing of rare targets, CamelBio streamlines your supply chain for diagnostic development.