GPR182 Rabbit pAb for WB, ELISA - O15218

Core Product Specifications and Parameters

Biological Background: GPR182/ACKR5 Function and Localization

• GPR182, also designated atypical chemokine receptor 5 (ACKR5), is a member of the G protein-coupled receptor superfamily characterized by seven transmembrane domains.
• Functions as an atypical chemokine receptor that regulates chemokine availability through scavenging and internalization without activating classical G protein-mediated signaling.
  Related references: PMID:33875597, PMID:35013216, PMID:37554323
• Exhibits broad ligand specificity, binding numerous chemokines from the CXC, CC, and XC subfamilies, including CXCL9, CXCL10, CXCL12, CXCL13, CCL19, CCL25, CCL28, and XCL1. It is the sole atypical receptor for CXCL13 and CCL28.
  Related references: PMID:33875597, PMID:35013216, PMID:38026988, PMID:37554323
• Displays strong constitutive association with beta-arrestins, which is essential for intracellular receptor trafficking and chemokine scavenging, occurring independently of ligand binding.
  Related references: PMID:33875597, PMID:37554323
• Cooperates with other atypical chemokine receptors ACKR3 and ACKR4 to modulate serum levels of CXCL12 and CCL19, respectively, highlighting its role in chemokine homeostasis.
  Related references: PMID:37554323
• Primarily localizes to the cell membrane and endosome membrane, consistent with its role in ligand internalization and recycling.
• Highly expressed in the heart, skeletal muscle, immune system, adrenal gland, and liver, indicating diverse physiological functions.
• Contains structural features such as disulfide bonds and N-linked glycosylation sites, and is classified under glycoprotein, transmembrane helix, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the cytoplasmic C-terminal region (amino acids 250–350) of human GPR182, suggesting the antibody may recognize multiple epitopes and detect both denatured and native conformations.
• For western blot (WB) analysis, the expected molecular weight of GPR182 is approximately 45 kDa; consider using appropriate positive controls such as lysates from heart or immune tissues where expression is high.
• For ELISA, optimize coating conditions and antibody dilutions; the antibody may be suitable for both direct and indirect ELISA formats.
• Cross-reactivity has been observed with mouse GPR182; validate reactivity in the specific experimental system if using other species.
• The antibody's polyclonal nature may lead to lot-to-lot variation; it is recommended to include appropriate negative controls and validate new lots in the intended assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, offering a streamlined pathway from concept to clinic. This anti-GPR182 polyclonal antibody is part of our extensive collection tailored for chemokine and immunology research. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized procurement for rare targets, all designed to support your diagnostic development with reliability and efficiency.