GPAM Rabbit Polyclonal Antibody for WB, ELISA - Q9HCL2

Core Product Specifications and Parameters

Biological Background: GPAM Function and Localization

• GPAM is a mitochondrial outer membrane protein that catalyzes the first and rate-limiting step in the biosynthesis of glycerolipids, including triglycerides and phospholipids. Related references: PMID:18238778, PMID:19075029, PMID:36522428

• This enzyme esterifies an acyl group from acyl-CoA to the sn‑1 position of glycerol‑3‑phosphate, forming lysophosphatidic acid. Related references: PMID:18238778

• GPAM possesses a narrow hydrophobic binding cleft selective for linear acyl chains. Related references: PMID:36522428

• It exhibits higher catalytic activity with 16‑carbon acyl‑CoA substrates. Related references: PMID:36522428

• The protein is located at the mitochondrial outer membrane.

• Classified by UniProt under lipid biosynthesis, phospholipid metabolism, and acyltransferase activity.

• Synonyms include GPAT1 and KIAA1560; post‑translational modifications include acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 594‑828), which may confer isoform or target specificity.
• For Western blotting, consider a 94 kDa predicted molecular weight when selecting gel percentage and transfer conditions.
• Validate the antibody in relevant sample systems (human, mouse, rat) to confirm cross‑reactivity and performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with seamless access to premium IVD raw materials, including this anti‑GPAM antibody for lipid metabolism studies. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare‑target sourcing to support your assay development from concept to clinic.