GP6 Rabbit Polyclonal Antibody for WB and ELISA - Q9HCN6

Core Product Specifications and Parameters

Biological Background: GP6 Function and Localization

• Platelet glycoprotein VI (GP6) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily.
• It functions as the major collagen receptor on platelets, mediating collagen-induced platelet adhesion, activation, and aggregation.
• Upon ligand binding, GP6 signals through the immunoreceptor tyrosine-based activation motif (ITAM)-coupled FcRγ chain, leading to activation of Src kinases, SYK, LAT, and PLCγ2.
• GP6 plays a pivotal role in platelet procoagulant activity, promoting thrombin generation and fibrin formation, thereby contributing to both arterial and venous thrombosis.
• The protein is predominantly expressed on the plasma membrane of megakaryocytes and platelets.
• Alternative splicing generates multiple GP6 isoforms.
• Post-translational modifications include glycosylation and disulfide bond formation.
• The protein contains an extracellular region with two immunoglobulin-like domains, a single transmembrane helix, and a short intracellular tail.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 21–267), which includes the ligand-binding immunoglobulin-like domains. This region is well-suited for detecting both full-length and soluble GP6 fragments.
• For Western blot, the predicted molecular weight of human GP6 is ~37 kDa; however, glycosylation may cause a shift in apparent molecular weight. Validate in platelet lysates or GP6-expressing cell lines.
• The antibody cross-reacts with mouse and rat GP6, enabling studies in rodent models of thrombosis and hemostasis.
• In ELISA, consider using recombinant GP6 extracellular domain as a positive control to assess binding.
• For immunohistochemistry or flow cytometry, note that this polyclonal antibody may recognize both native and denatured GP6; optimization is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers working on platelet function and thrombosis. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, and can assist in sourcing rare-target raw materials like GP6 antibodies for your assay development.