GORASP2 Rabbit pAb for WB, IF/ICC, ELISA - Q9H8Y8

Core Product Specifications and Parameters

Biological Background: GORASP2 Function and Localization

• GORASP2 (also known as GRASP55, GOLPH6, p59, GRS2) is a key structural protein of the Golgi apparatus that interacts with GORASP1/GRASP65 to form and maintain the Golgi ribbon. Related references: PMID:33301566
• It plays a role in the assembly and membrane stacking of Golgi cisternae, particularly during the reassembly of Golgi stacks after mitosis and meiosis. Related references: PMID:10487747 PMID:21515684 PMID:22523075
• Mediates ER stress-induced unconventional trafficking of core-glycosylated CFTR to the plasma membrane, bypassing the Golgi. Related references: PMID:21884936 PMID:27062250 PMID:28067262
• Regulates the intracellular transport and cell surface presentation of transmembrane proteins such as TGFA. Related references: PMID:11101516
• Required for normal acrosome formation during spermiogenesis and male fertility, likely by facilitating JAM2 and JAM3 localization at germ cell–Sertoli cell contact sites.
• GORASP2 is localized to the Golgi apparatus membrane and endoplasmic reticulum membrane, and is subject to phosphorylation, myristoylation, palmitoylation, and methylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 353-452) of human GORASP2; based on cross-reactivity data, this region is conserved in mouse and rat.
• For western blotting, reduce and denature samples; expect a band around 47 kDa. Use appropriate positive controls (e.g., HeLa or HEK293 cell lysates) and validate specificity in your system.
• For immunofluorescence/immunocytochemistry, fix cells with paraformaldehyde and permeabilize; anticipate Golgi-like perinuclear staining. Co-staining with a Golgi marker (e.g., GM130) is recommended to confirm localization.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical assays; titrate the antibody for each new application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For Golgi biology and intracellular trafficking targets like GORASP2, we offer one-stop sourcing from discovery to validation. Contact us to discuss custom antibody development, rare target procurement, or technical integration services.