GNS Rabbit Polyclonal Antibody for WB and ELISA - P15586

Core Product Specifications and Parameters

Biological Background: GNS (N-acetylglucosamine-6-sulfatase) Function and Localization

• GNS encodes N-acetylglucosamine-6-sulfatase (also known as glucosamine-6-sulfatase), a lysosomal enzyme that hydrolyzes 6-sulfate groups from N-acetyl-D-glucosamine units in heparan sulfate and keratan sulfate.
• This enzyme is essential for the stepwise degradation of glycosaminoglycans; its deficiency leads to mucopolysaccharidosis type IIID (Sanfilippo D syndrome), a lysosomal storage disorder.
• The full-length protein contains a signal peptide that directs it to the lysosome, where it exerts its catalytic function.
• Alternative splicing generates multiple isoforms; the protein undergoes post-translational modifications such as glycosylation and phosphorylation, and binds calcium ions.
• GNS is broadly expressed across human tissues, as evidenced by proteomic identification, consistent with its housekeeping role in lysosomal catabolism.
• UniProt keywords: Alternative splicing, Calcium, Disease variant, Glycoprotein, Hydrolase, Lysosome, Metal-binding, Mucopolysaccharidosis, Phosphoprotein, Signal.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 293–552 of human GNS, a region conserved in mouse and rat orthologs; verify reactivity in your specific experimental system.
• For Western blot, a band of approximately 62 kDa is expected based on the predicted molecular weight of full-length GNS.
• Optimize antibody dilution empirically for ELISA; the polyclonal nature may provide robust signal detection.
• Consider including appropriate controls, such as lysates from cell lines expressing GNS or siRNA-treated cells, to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For glycobiology and lysosomal storage disorder research applications, our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you are developing assays for GNS activity or screening for mucopolysaccharidosis biomarkers, CamelBio can support your raw material requirements from development through scale-up.