GnRH1 Rabbit Polyclonal Antibody for WB and ELISA - P01148

Core Product Specifications and Parameters

Biological Background: GnRH1 Function and Localization

• GnRH1, also known as progonadoliberin-1, is a precursor protein that is processed to produce gonadotropin-releasing hormone 1 (GnRH), a key reproductive hormone.
• GnRH is a decapeptide hormone that stimulates the anterior pituitary gland to secrete luteinizing hormone (LH) and follicle-stimulating hormone (FSH), critical for gonadal function.
• The protein is synthesized as a preproprotein and undergoes proteolytic cleavage on pairs of basic residues to generate the active hormone, which is then amidated and has a pyroglutamic acid at its N-terminus.
• GnRH1 is a secreted protein, belonging to the GnRH family, and plays a central role in the hypothalamic-pituitary-gonadal axis.
• Mutations in the GNRH1 gene are associated with reproductive disorders such as hypogonadotropic hypogonadism and Kallmann syndrome.
• As a pharmaceutical target, GnRH analogs are used for conditions like endometriosis, prostate cancer, and precocious puberty.
• Keywords: Secreted, hormone, amidation, cleavage on pair of basic residues, pyrrolidone carboxylic acid, disease variant.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide spanning the N-terminal region (aa 1-92) of human GNRH1, the antibody likely recognizes the proprotein form; consider this when designing assays.
• For western blot, the predicted molecular weight is approximately 10 kDa; ensure appropriate gel percentage and transfer conditions for low molecular weight proteins.
• Cross-reactivity with mouse is reported; validate in your experimental system if studying mouse models.
• For ELISA, the antibody may capture both pro-GnRH and mature GnRH; appropriate controls are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibodies and reagents for reproductive endocrinology research. This GnRH1 rabbit polyclonal antibody exemplifies our commitment to providing high-quality, ready-to-use tools for diagnostic development. From optimized antibody pairs to bulk ancillary reagents, we support your entire workflow from concept to clinic.