GNE Rabbit Monoclonal Antibody for WB, ELISA - Q9Y223

Core Product Specifications and Parameters

Biological Background: GNE Function and Localization

• GNE encodes a bifunctional enzyme possessing UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, catalyzing the rate-limiting step in the biosynthesis of sialic acids (PubMed:10334995, PubMed:11326336, PubMed:14707127).
  Related references:
  PMID:10334995, PMID:11326336, PMID:14707127
• Sialic acids are negatively charged terminal sugars on cell surface glycoconjugates that mediate critical cellular processes including adhesion, signal transduction, and recognition.
• The enzyme is localized to the cytoplasm/cytosol.
• Alternative splicing generates multiple isoforms with tissue-specific expression: isoform 1 is broadly expressed, isoform 2 is predominantly found in placenta, and isoform 3 is expressed at low levels in kidney, liver, placenta, and colon.
• Highest overall GNE expression is observed in liver and placenta, with additional expression in heart, brain, lung, kidney, skeletal muscle, and pancreas.
• GNE is an allosteric enzyme and a phosphoprotein, requiring zinc metal binding and ATP for its kinase function.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa 623-722) lies in the C-terminal region of human GNE; sequence alignment with rat GNE supports the reported cross-reactivity.
• For Western blotting, consider standard denaturing SDS-PAGE and transfer to PVDF or nitrocellulose membrane. The predicted molecular weight is ~79 kDa, though post-translational modifications may cause slight band shifts.
• For ELISA applications, coating with the immunogen peptide or recombinant GNE protein is recommended; validate assay conditions in your specific sample matrix.
• If using this antibody in functional studies, verify that it does not interfere with GNE enzymatic activity, as it targets the C-terminal domain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, clinical laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, supporting the entire development pipeline from concept to clinic. This GNE Rabbit mAb exemplifies our commitment to providing validated antibodies for glycosylation and metabolic enzyme research, including rare targets like sialic acid biosynthesis regulators. Beyond individual antibodies, we offer matched antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized raw-material sourcing to meet your assay development and production needs.