GNAS Rabbit pAb for WB, IF/ICC, IP, ELISA - O95467 / P63092 / P84996 / Q5JWF2

Core Product Specifications and Parameters

Biological Background: GNAS Function and Localization

• The GNAS gene encodes multiple proteins through alternative splicing, including the neuroendocrine secretory protein 55 (NESP55), which is derived from the O95467 entry.
• NESP55 is a secreted proteoglycan that localizes to cytoplasmic secretory vesicles and is released via the regulated secretory pathway in neuroendocrine cells.
• This protein is involved in neuroendocrine secretion and may play a role in hormone trafficking and release, as indicated by its presence in secretory vesicles.
• Subcellularly, NESP55 is found in cytoplasmic vesicles and is ultimately secreted, suggesting a function in intercellular signaling or modulation of the extracellular environment.
• The GNAS locus also produces other signaling proteins, such as the stimulatory G protein alpha subunit (Gsα), highlighting the complex regulation of this imprinted gene region.
• Mutations or dysregulation of GNAS products are associated with disorders like pseudohypoparathyroidism, McCune-Albright syndrome, and Cushing syndrome, underscoring the clinical relevance of GNAS-related pathways.
• NESP55 is characterized by a signal peptide, a pair of basic residues cleavage site, and glycosylation, which are typical features of secretory granule proteins.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 1–100 of human GNAS (isoform XLas), which may confer reactivity with multiple GNAS isoforms depending on sequence homology.
• For Western blot, multiple bands may be observed due to the various protein weights of GNAS splice variants (28, 44, 45, 77, 109, 111 kDa); consider using isoform-specific controls.
• In immunofluorescence/immunocytochemistry, the antibody may detect NESP55 in secretory vesicles or other GNAS products at the plasma membrane depending on the cell type.
• For immunoprecipitation, rabbit polyclonal antibodies are compatible with Protein A/G beads; ensure appropriate antibody-bead ratios for target enrichment.
• ELISA applications may require optimization of coating antigen and detection conditions; validate in the relevant sample system before quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to high-quality IVD raw materials like the GNAS rabbit polyclonal antibody, backed by expertise in antibody development and validation. As your sourcing partner, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement to accelerate your neuroendocrine or signaling-related diagnostic projects. With our comprehensive support from concept to clinic, you can focus on assay development while we handle the supply chain.