GNAI2 Rabbit mAb for WB, IHC-P, ELISA - P04899

Core Product Specifications and Parameters

Biological Background: GNAI2 Function and Localization

• GNAI2 encodes the guanine nucleotide-binding protein G(i) subunit alpha-2, which acts as a transducer in GPCR signaling cascades, coupling receptor activation to intracellular effectors [PMID:38505600, 29925945, 35637350].
  Related references: PMID:38505600, PMID:29925945, PMID:35637350
• The alpha subunit alternates between an inactive GDP-bound state and an active GTP-bound state; upon GPCR activation, GDP is released and replaced by GTP, triggering downstream signaling [PMID:12359238, 29925945].
  Related references: PMID:12359238, PMID:29925945
• GNAI2 inhibits adenylate cyclase activity, reducing cellular cAMP levels, and mediates inhibitory regulation of hormone-sensitive adenylate cyclase in response to beta-adrenergic stimuli.
• In endothelial cells, GNAI2 plays an important role in activating the transcription factor NFAT to promote angiogenesis [PMID:38505600].
  Related references: PMID:38505600
• GNAI2 is essential for neutrophil recruitment during acute inflammation, facilitating polymorphonuclear neutrophil transmigration (By similarity).
• It regulates the cell surface density of dopamine receptors DRD2 by sequestering them as an intracellular pool.
• GNAI2 localizes to the cytoplasm, centrosome, and cell membrane, reflecting its roles in signal transduction and cytoskeletal interactions.
• The protein is highly expressed in endothelial cells and undergoes lipid modifications (myristoylation and palmitoylation) that anchor it to membranes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250–355 of human GNAI2; therefore, the antibody is likely to detect a linear epitope and is well-suited for WB under denaturing and reducing conditions.
• For IHC-P, consider using heat-induced antigen retrieval in citrate buffer (pH 6.0) to expose the epitope in formalin-fixed paraffin-embedded tissues.
• Validate antibody specificity using appropriate positive (e.g., human endothelial cells or tissues with high GNAI2 expression) and negative controls; note the predicted molecular weight of ~40 kDa on Western blots.
• Optimize antibody dilutions for each application and sample type; a starting range of 1:500–1:2000 for WB and 1:50–1:200 for IHC-P is commonly used, but empirical determination is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with comprehensive access to IVD raw materials, technical services, and expert consulting. This GNAI2 antibody is part of our expanding portfolio for GPCR signaling and inflammation research. We provide validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to streamline your assay development from concept to clinic.