GNA14 Rabbit pAb - O95837

Core Product Specifications and Parameters

Biological Background: GNA14 Function and Localization

• GNA14 encodes a guanine nucleotide-binding protein (G protein) alpha subunit that acts as a modulator or transducer in various transmembrane signaling systems.
• As a G protein alpha subunit, it functions as a molecular switch by cycling between GTP-bound active and GDP-bound inactive states, enabling signal relay from cell surface receptors to intracellular effectors.
• The protein requires magnesium ions as a cofactor for nucleotide binding and hydrolysis, and is classified under metal-binding and GTP-binding keywords.
• GNA14 is subject to ADP-ribosylation, a key post-translational modification that can regulate its activity and interactions.
• The observed molecular weight is approximately 42 kDa, consistent with its predicted mass of 41.6 kDa, and it is detectable across human, mouse, and rat samples.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 40–350 of human GNA14, covering a large portion of the protein, which suggests the antibody may recognize multiple epitopes and be suitable for detection applications.
• For Western blot, a band at ~42 kDa is expected; consider using appropriate positive controls (e.g., cell lines expressing GNA14) and optimizing blocking/detection conditions.
• In immunofluorescence (IF/ICC), validate the antibody in relevant human, mouse, or rat cell lines, and include permeabilization steps to access intracellular GNA14.
• For ELISA, the polyclonal nature may allow use as either capture or detection reagent; test direct and sandwich formats with recombinant or native protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic developers and research labs by providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized raw material sourcing for rare targets. For GNA14 and other G protein signaling components, we help you secure reliable, high-quality raw materials from concept to clinic, enabling seamless assay development and scale-up.