GNA12 Rabbit Polyclonal Antibody (pAb) for WB, IHC-P, ELISA - Q03113

Core Product Specifications and Parameters

Biological Background: GNA12 Function and Localization

• GNA12 encodes guanine nucleotide-binding protein subunit alpha-12, a key modulator or transducer in various transmembrane signaling systems. Related references: PMID:12515866, PMID:15240885, PMID:15525651
• GNA12 activates the RhoA signaling pathway by binding and activating RhoGEFs such as ARHGEF12/LARG. Related references: PMID:12515866, PMID:15240885, PMID:16202387
• GNA12-dependent Rho signaling regulates protein phosphatase 2A (PP2A) activation, leading to dephosphorylation of target proteins. Related references: PMID:15525651, PMID:17565996
• GNA12 promotes tumor cell invasion and metastasis through RhoA/ROCK signaling and upregulation of pro-inflammatory cytokines. Related references: PMID:16705036, PMID:16787920, PMID:23762476
• GNA12 inhibits CDH1-mediated cell adhesion independently of Rho activation and, together with NAPA, promotes CDH5 localization to the plasma membrane. Related references: PMID:11976333, PMID:16787920, PMID:15980433
• GNA12 may play a role in cell migration control through the TOR signaling cascade. Related references: PMID:22609986
• GNA12 is localized at the cell membrane, lateral cell membrane, and cytoplasm.
• GNA12 undergoes post-translational modifications including palmitoylation and phosphorylation, and alternative initiation generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 285-381), which may allow detection of both full-length and potential alternative initiation isoforms.
• For Western blot (WB), the expected band is approximately 44 kDa; validate the antibody using positive control lysates from human, mouse, or rat tissues given the cross-reactivity.
• For immunohistochemistry (IHC-P), consider optimized antigen retrieval methods and test in tissues with known GNA12 expression.
• For ELISA, pair with an appropriate capture antibody or antigen coating to assess binding specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This GNA12 antibody is part of our extensive catalog of validated antibodies and ancillary reagents for G protein signaling research. In addition to ready-to-use products, we support custom antibody development, bulk reagent supply, and rare target sourcing to accelerate your diagnostic and research timelines.