GMNN Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - O75496

Core Product Specifications and Parameters

Biological Background: GMNN Function and Localization

• DNA replication inhibitor: Inhibits DNA replication by preventing incorporation of the MCM complex into the pre-replication complex (pre-RC). Related references: PMID:14993212, PMID:20129055, PMID:24064211
• Cell cycle-regulated degradation: Degraded during the mitotic phase of the cell cycle; destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Related references: PMID:14993212, PMID:24064211, PMID:9635433
• Histone acetyltransferase inhibition: Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, thereby inhibiting histone H4 acetylation and DNA replication licensing. Related references: PMID:20129055
• Hox protein transcriptional inhibition: Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control. Related references: PMID:22615398
• Subcellular localization: Localizes to both the cytoplasm and nucleus; nuclear accumulation is cell-cycle dependent.
• Disease association: Mutations in GMNN cause Meier-Gorlin syndrome type 6 (MGORS6), a primordial dwarfism disorder.
• Post-translational modifications: Subject to acetylation and phosphorylation; contains a coiled-coil domain.

Experimental Guidance and Technical Tips

• The immunogen (full-length human GMNN, amino acids 1–209) suggests the antibody recognizes total GMNN; consider its suitability for detecting multiple isoforms or cleavage products.
• For WB, expect a band at ~24 kDa; using nuclear and cytoplasmic extracts may help confirm localization-dependent expression.
• For IF/ICC, validate in cell lines with known GMNN expression; note that GMNN levels fluctuate during the cell cycle, with highest nuclear signals in S/G2 phases.
• For IHC-P, optimize antigen retrieval; expect predominantly nuclear staining in proliferative regions; include appropriate controls to confirm specificity.
• ELISA applications may require further validation for quantitative detection; consider pairing with a capture antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing experience for IVD raw materials, connecting you with validated antibodies, recombinant proteins, and ancillary reagents for diagnostic and research applications. This GMNN polyclonal antibody supports studies on DNA replication licensing and cell cycle control. For your IVD development pipeline, CamelBio provides bulk antibody supplies, customized antibody pair screening, and sourcing support for niche targets, ensuring continuity from concept to clinic.