GM130 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q08379

Core Product Specifications and Parameters

Biological Background: GM130/GOLGA2 Function and Localization

• GM130, encoded by the GOLGA2 gene, is a 130 kDa cis-Golgi matrix protein also known as golgin-95 and GM130 autoantigen (UniProt Q08379).
• Acts as a peripheral membrane component of the cis-Golgi, maintaining Golgi stack structure and facilitating vesicle tethering and fusion to the Golgi membrane (PubMed:16489344).
  Related references:
  PMID:16489344
• Together with p115/USO1 and STX5, it mediates vesicle docking and fusion to maintain an interconnected, stacked Golgi apparatus (By similarity).
• Plays a critical role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 during mitosis disrupts interaction with p115, inhibiting COPI vesicle tethering and Golgi transport (By similarity).
• Also essential for spindle pole assembly and centrosome organization; upon mitotic phosphorylation, it recruits importin-α to Golgi membranes, releasing TPX2 to activate AURKA and nucleate microtubules, thereby linking mitotic membranes to the spindle (PubMed:19242490, PubMed:26165940).
  Related references:
  PMID:19242490, PMID:26165940
• Required for Golgi ribbon formation and efficient glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).
  Related references:
  PMID:16489344, PMID:17314401
• Localizes primarily to the cis-Golgi network membrane and the endoplasmic reticulum-Golgi intermediate compartment, and under mitotic conditions also associates with the spindle pole and cytoskeleton.
• Annotated with keywords including Alternative splicing, Cell cycle, Cell division, Coiled coil, Phosphoprotein, and Microtubule, reflecting its diverse regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human GM130; the antibody may preferentially detect targets containing this N-terminal region.
• For Western blotting, a band at approximately 113–130 kDa is expected; include a known positive control (e.g., HeLa or HEK293 cell lysate) and adjust antibody dilution accordingly.
• In immunofluorescence/ICC, anticipate perinuclear cis-Golgi staining; co-staining with a cis-Golgi marker (e.g., GM130 itself as a known marker) can aid interpretation.
• For immunohistochemistry (IHC-P), test antigen retrieval methods and optimize primary antibody concentration using tissues with known GM130 expression.
• The antibody is also suitable for ELISA; users may consider sandwich ELISA designs using paired antibodies if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with one-stop access to a broad portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents. For GM130/GOLGA2 research, we offer rabbit monoclonal antibodies and supporting reagents to accelerate assay development for Golgi biology and cell division studies. Beyond catalog products, our flexible sourcing network assists in procuring rare targets and optimizing raw-material supply chains from concept to clinic.