Glycogen synthase 1 (GYS1) Rabbit Monoclonal Antibody for WB, ELISA - P13807

Core Product Specifications and Parameters

Biological Background: Glycogen synthase 1 (GYS1) Function and Localization

• Glycogen synthase 1 (GYS1), also known as muscle glycogen synthase, is the key enzyme in glycogen biosynthesis, catalyzing the transfer of glucosyl residues from UDP-glucose to the non-reducing end of alpha-1,4-glucan primers.
• It works in concert with glycogenin (which initiates the glycogen primer) and glycogen branching enzyme to extend and branch the glycogen molecule.
• GYS1 is expressed predominantly in skeletal muscle, but also in most cell types where glycogen serves as an energy reservoir.
• The enzyme is subject to allosteric regulation and reversible phosphorylation, which modulate its activity in response to insulin and metabolic signals.
• Alternative splicing of the GYS1 transcript produces multiple isoforms, which may differ in regulatory properties.
• Mutations and dysregulation of GYS1 are implicated in metabolic disorders, including diabetes mellitus, as highlighted by UniProt disease variant annotations.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 638–737) lies within the C-terminal region of human GYS1, a domain that is conserved among mammals; antibody reactivity is therefore expected across human, mouse, and rat species.
• For Western blotting, use reducing SDS-PAGE and load 20–50 µg of total protein from skeletal muscle, liver, or cell line lysates known to express GYS1. The predicted band size is approximately 84 kDa.
• When performing ELISA, consider using a paired antibody format if developing a sandwich assay, and validate the antibody’s performance with recombinant GYS1 protein or cell lysates to confirm sensitivity and specificity.
• Since GYS1 is a phosphoprotein, treatment of samples with phosphatase inhibitors may be necessary to preserve phosphorylation states if those are targets of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, supporting your entire workflow from concept to clinic. For research into glycogen metabolism, muscle function, and diabetes, we offer high-quality monoclonal antibodies like this anti-GYS1 clone, along with validated antibody pairs, ancillary reagents, and custom sourcing for rare targets. Let CamelBio streamline your assay development with reliable materials and expert technical support.