Glutathione Peroxidase 2 Rabbit Monoclonal Antibody for WB and ELISA - P18283

Core Product Specifications and Parameters

Biological Background: Glutathione Peroxidase 2 Function and Localization

• Glutathione Peroxidase 2 (GPX2), also known as gastrointestinal glutathione peroxidase, GPx-GI, GSHPx-GI, GPRP-2, and phospholipid hydroperoxide glutathione peroxidase, is a member of the glutathione peroxidase family that primarily functions in cellular antioxidant defense.
• GPX2 catalyzes the glutathione-dependent reduction of hydroperoxides, including H2O2, cumene hydroperoxide, tert-butyl hydroperoxide, and several fatty acid-derived hydroperoxides, thereby regulating cellular redox homeostasis; it does not reduce phosphatidylcholine hydroperoxide. Related references: PMID:36608588 PMID:8428933
• The protein localizes to the cytoplasm and cytosol, consistent with its role in intracellular peroxide detoxification.
• Expression is tissue-specific, predominantly detected in the liver and gastrointestinal tract, with absence in heart and kidney, underscoring its specialized role in digestive system redox balance.
• GPX2 contains a selenocysteine (Sec) residue at its active site, which is essential for its peroxidase activity and is encoded by a recoded UGA codon.
• As a reference proteome member with a known 3D structure, GPX2 is a well-characterized enzyme in oxidative stress and inflammation research, particularly in gastrointestinal pathologies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 70–174) of human GPX2, which avoids predicted transmembrane or heavily modified domains; consider verifying antibody specificity using GPX2-overexpressing cell lysates or siRNA knockdown in a relevant gastrointestinal cell line.
• For Western blot, a reducing SDS-PAGE is recommended; the expected band size is approximately 22 kDa, but may shift slightly depending on post-translational modifications or sample preparation.
• For ELISA, pair this antibody with a matched detection antibody recognizing a non-overlapping epitope; validate the sandwich assay using recombinant human GPX2 or native tissue extracts.
• Since GPX2 expression is high in liver and gut, use HepG2, Caco-2, or tissue homogenates as positive controls; heart or kidney lysates can serve as negative controls.
• Optimize primary antibody dilution empirically, starting from 1:500 to 1:2000 for WB and 0.5-2 µg/mL for ELISA, and include appropriate secondary detection reagents.
• Cross-reactivity with other glutathione peroxidases (e.g., GPX1, GPX3) has not been reported, but confirm specificity in your assay by probing recombinant family members if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner in IVD raw-material sourcing, accelerating the transition from concept to clinic. With the Glutathione Peroxidase 2 Rabbit Monoclonal Antibody, we support redox biology and gastrointestinal disease research by offering validated monoclonal antibodies, antibody pairs, and ancillary reagents. Beyond this product, our platform connects diagnostic manufacturers and laboratories with one-stop access to rare-target raw materials, optimized bulk antibodies, and technical consultation to streamline your assay development.