Glutaminase (GLS) Rabbit mAb for WB, IF/ICC, IF-P, ELISA - O94925

Core Product Specifications and Parameters

Biological Background: Glutaminase (GLS) Function and Localization

• Glutaminase (GLS) catalyzes the first step in renal glutamine catabolism, playing a key role in acid-base homeostasis and regulation of the neurotransmitter glutamate. Related references: PMID:30239721 PMID:30575854 PMID:30970188
• The protein exists in multiple isoforms generated by alternative splicing; isoform 1 (kidney-type) and isoform 3 are catalytically active, while isoform 2 (lacks the catalytic domain) is found in cardiac and skeletal muscle and is inactive.
• Isoform 1 is predominantly expressed in brain and kidney, isoform 3 in heart and pancreas, and isoform 2 in cardiac and skeletal muscle.
• GLS localizes primarily to the mitochondrial matrix, but can also be found in the cytoplasm and cytosol.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and interaction partners.
• Mutations in GLS are associated with diseases such as epilepsy and cataracts, highlighting its importance in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 40-140, which may target both active isoforms 1 and 3. For WB, a band near 73 kDa is expected; consider validation in positive control lysates such as kidney or brain.
• For IF/ICC, co-staining with mitochondrial markers is recommended to confirm the expected mitochondrial localization. Mouse and rat cross-reactivity is reported; verify in relevant tissue samples.
• For ELISA, the peptide-based immunogen target can be used for detection; optimize antibody concentration according to the specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, including high-quality monoclonal antibodies like this anti-GLS antibody. Our platform connects diagnostic developers with validated antibody pairs, bulk reagents, and rare target sourcing—supporting your journey from concept to clinic. Whether you require optimized antibodies for glutamine metabolism research or ancillary reagents for assay development, CamelBio is your trusted partner.