Gli3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P10071

Core Product Specifications and Parameters

Biological Background: GLI3 Function and Localization

• GLI3 encodes a key transcription factor of the Hedgehog signaling pathway, essential for patterning of the brain, limbs, and other body structures. Related references: PMID:10075717 PMID:10375510 PMID:10693759
• The full-length GLI3 protein (GLI3FL/GLI3-190, ~190 kDa) acts as a transcriptional activator upon Smoothened (SMO) activation, but is constitutively processed into the major repressor form (GLI3R) in the absence of Hedgehog signaling. Related references: PMID:10375510 PMID:10693759
• GLI3 specifically recognizes the consensus DNA sequence 5'-GACCACCCA-3' to directly regulate target gene expression. Related references: PMID:10075717
• The protein dynamically localizes to the nucleus, cytoplasm, and primary cilium, reflecting its role in signal transduction.
• Post-translational modifications include acetylation, phosphorylation, methylation, and ubiquitination, which fine-tune its stability and activity (UniProt keywords: Acetylation, Phosphoprotein, Methylation, Isopeptide bond, Ubl conjugation).
• Mutations in GLI3 are linked to developmental disorders such as Greig cephalopolysyndactyly syndrome (GCPS) and Pallister-Hall syndrome (PHS) (UniProt: Disease variant).
• It is broadly expressed in adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.

Experimental Guidance and Technical Tips

• The immunogen (aa 1301–1580) lies within a region downstream of the zinc finger domains; confirm that the epitope is present in both full-length and processed isoforms if studying cleavage products.
• For Western blot, the predicted molecular weight is ~170 kDa; prolonged electrophoresis and transfer may be required due to the large protein size.
• Validate the antibody in your sample system (human, mouse, or rat) using appropriate positive controls (e.g., cell lines with known GLI3 expression or Hedgehog pathway activation).
• In immunofluorescence/ICC, anticipate nuclear, cytoplasmic, or ciliary staining patterns depending on signaling status.
• ELISA can be used for quantitative detection, but optimal coating and detection conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, simplifying raw-material procurement from discovery to commercialization. Our portfolio includes this high-quality GLI3 rabbit monoclonal antibody, ideal for Hedgehog pathway and developmental biology investigations. Beyond this product, we offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and customized sourcing for rare targets—all designed to streamline your assay development and scale-up processes.