GLB1 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P16278

Core Product Specifications and Parameters

Biological Background: GLB1 Function and Localization

• GLB1 (acid beta-galactosidase, elastin receptor 1) is a lysosomal enzyme that hydrolyzes terminal beta-linked galactose residues from glycoconjugates.
• Beyond its catalytic function, GLB1 also acts as the elastin-binding protein (EBP), a molecular chaperone that facilitates tropoelastin secretion and elastic fiber assembly in connective tissues.
• Subcellular localization is primarily lysosomal, with additional detection in the cytoplasm and perinuclear region.
• It is a glycosylated, disulfide-linked protein synthesized as a zymogen that requires proteolytic activation.
• Tissue specificity: expressed in placenta, fibroblasts, and testis. Related references: PMID:8383699 PMID:2511208
• Mutations in GLB1 cause GM1 gangliosidosis and Morquio B syndrome (mucopolysaccharidosis IVB).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 329–677 of human GLB1, covering the C-terminal domain; it may detect both full-length and truncated forms. For WB, a band around 76 kDa is expected, while alternative splicing or post-translational processing could yield additional bands.
• For IF/ICC and IHC-P, validate subcellular staining patterns (lysosomal/perinuclear) using known positive controls such as fibroblasts or placental tissue.
• The antibody cross-reacts with mouse and rat, making it suitable for cross-species studies; confirm reactivity in your model system with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This GLB1 antibody supports research into lysosomal storage disorders and connective tissue biology, and we offer customized sourcing solutions to accelerate your assay development.