GEMIN7 Rabbit Polyclonal Antibody for WB and ELISA - Q9H840

Core Product Specifications and Parameters

Biological Background: GEMIN7 Function and Localization

• GEMIN7, also known as SIP3, is a component of the survival motor neuron (SMN) complex, which is essential for the assembly of small nuclear ribonucleoproteins (snRNPs).
• The SMN complex catalyzes the assembly of the Sm core on pre-snRNA, a critical step in the biogenesis of spliceosomal snRNPs that mediate pre-mRNA splicing.
• In the cytoplasm, the SMN complex interacts with the pICln-Sm protein complex to facilitate the transfer of Sm proteins to snRNA, forming the core snRNP.
• GEMIN7 is localized in the nucleus (nucleoplasm and gems) and the cytoplasm, consistent with its role in snRNP assembly occurring in both compartments.
• Post-translational modifications of GEMIN7 include acetylation and phosphorylation, which may regulate its function or interactions within the SMN complex.
• Belongs to the gemin family of proteins, which are key components of the SMN complex involved in RNA processing, particularly mRNA splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-131) of human GEMIN7; consider this epitope location when designing detection or blocking strategies.
• For Western blot, the predicted molecular weight is 15 kDa; optimize gel percentage and transfer conditions for low-molecular-weight targets, and use appropriate molecular weight markers.
• In ELISA applications, direct coating with the immunogen or recombinant GEMIN7 protein may serve as a positive control; validate with your specific antigen format.
• As a polyclonal antibody, potential lot-to-lot variability exists; include appropriate controls and validate performance in your experimental system.
• Reactivity is confirmed with human samples; cross-reactivity with other species is not specified, so validate if used with non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, serving diagnostic manufacturers, labs, and research institutes. This anti-GEMIN7 polyclonal antibody supports studies on snRNP assembly and pre-mRNA splicing, and can be sourced alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. With end-to-end technical and consulting support, CamelBio bridges the gap from concept to clinic in your diagnostic development pipeline.