GCN2 Rabbit mAb - Q9P2K8

Core Product Specifications and Parameters

Biological Background: GCN2 Function and Localization

• GCN2 (eIF-2-alpha kinase 4, also known as GCN2-like protein) is a member of the eIF-2-alpha kinase family, functioning as a key metabolic-stress sensor.
• Phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha) in response to amino acid starvation, leading to global attenuation of cap-dependent translation while promoting translation of stress-responsive mRNAs such as ATF4. Related references: PMID:25329545, PMID:32610081
• Acts as an activator of the integrated stress response (ISR), essential for cellular adaptation to amino acid deprivation.
• Modulates cell cycle progression by repressing cyclin D1 mRNA translation upon unfolded protein response (UPR) activation and by stimulating CDKN1A/p21 mRNA translation during amino acid scarcity. Related references: PMID:26102367
• Localizes predominantly to the cytoplasm.
• Widely expressed across tissues, with notable expression in lung, smooth muscle cells, and macrophages. Related references: PMID:10504407, PMID:24292273
• Possesses antiviral functions: impairs early viral mRNA translation during alphavirus infection (By similarity) and orchestrates dendritic cell-mediated adaptive immunity against yellow fever virus under amino acid starvation. Related references: PMID:24310610
• Subject to post-translational modifications including acetylation and phosphorylation, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• For Western blot (WB), account for the large protein size (~187 kDa); consider using a low-percentage SDS-PAGE gel (e.g., 6–8%) and extended transfer times.
• Validate antibody performance in positive control lysates from human, mouse, or rat cell lines known to express GCN2.
• For immunohistochemistry paraffin (IHC-P) and ELISA, optimize antigen retrieval and assay conditions for detection of cytoplasmic GCN2.
• Given that the immunogen spans the N-terminal region (aa 1–300), confirm that the antibody recognizes full-length and potential splice isoforms in relevant biological samples.
• Use appropriate molecular weight markers and loading controls for high-molecular-weight protein analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our portfolio supports research into translation regulation, stress responses, and kinase signaling pathways, with this GCN2 Rabbit mAb addressing key needs for studying integrated stress response mechanisms. We also offer specialized sourcing services for rare-target antibodies and custom reagent development, helping to streamline your workflow from concept to clinic.