GCET2 Rabbit Monoclonal Antibody for WB and ELISA - Q8N6F7

Core Product Specifications and Parameters

Biological Background: GCET2 Function and Localization

• GCET2, also known as HGAL (human germinal center-associated lymphoma) and GCAT2, is encoded by the GCSAM gene. It is specifically expressed in germinal center B-cells and lymphomas, playing a critical role in B-cell biology.
• The protein functions as a negative regulator of lymphocyte motility. It mediates the migration-inhibitory effects of interleukin-6 (IL-6), thereby controlling lymphocyte trafficking within lymphoid organs.
• GCET2 positively regulates the RhoA signaling pathway, enhancing RhoA activation and subsequent ROCK kinase activity. This leads to inhibition of lymphocyte and lymphoma cell motility.
• As a regulator of B-cell receptor signaling, GCET2 acts through SYK kinase activation, influencing downstream signaling events in B-cells.
• Subcellularly, GCET2 localizes to both the cytoplasm and cell membrane, indicating dynamic distribution that may be critical for its signaling functions.
• The protein undergoes alternative splicing and is post-translationally modified by phosphorylation and ubiquitin conjugation (Ubl), as indicated by Phosphoprotein and Ubl conjugation keywords.
• Tissue expression is prominent in normal germinal center lymphocytes, thymus, spleen, and germinal center-derived malignancies, including diffuse large B-cell lymphoma (DLBCL).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–178) of human GCET2, which is conserved across species; thus, the antibody may detect human, mouse, and rat GCET2 in Western blot and ELISA. Validate cross-reactivity in your assay system.
• For Western blot, a band at approximately 21 kDa is expected. Due to potential post-translational modifications or isoform variability, additional bands might appear; verify with appropriate controls.
• In ELISA, consider using recombinant GCET2 protein or cell lysates from germinal center B-cell lines as antigen; optimize coating and detection conditions experimentally.
• The antibody is produced in rabbit as a monoclonal reagent, offering batch-to-batch consistency for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a reliable bridge to high-quality IVD raw materials. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare-target sourcing, our platform supports your projects in immunology, oncology, and B-cell biology. This GCET2 antibody exemplifies our commitment to delivering precise tools for germinal center-related research and lymphoma diagnostics.