GBF1 Rabbit pAb - Q92538

Core Product Specifications and Parameters

Biological Background: GBF1 Function and Localization

• GBF1 acts as a guanine-nucleotide exchange factor (GEF) for Arf family GTPases, initiating vesicle coating by generating activated ARFs through GDP-to-GTP exchange. It is recruited to cis-Golgi membranes in an Arf-GDP-dependent manner and regulated by ARF1, ARF3, ARF4, and ARF5. Related references: PMID:12047556, PMID:12808027, PMID:16926190
• It is essential for the recruitment of the COPI coat complex to ER exit sites, ER-Golgi intermediate compartment (ERGIC), and cis-Golgi, and mediates COPI vesicle-dependent retrograde transport from these compartments back to the ER. Related references: PMID:12047556, PMID:12808027, PMID:16926190
• In the trans-Golgi network, GBF1 recruits adaptors GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 complex, facilitating clathrin-dependent transport; this function likely requires GEF activity toward ARF4 and ARF5. Related references: PMID:23386609
• It is required for Golgi apparatus assembly, and its phosphorylation by AMPK triggers Golgi disassembly during mitosis and stress. Related references: PMID:12808027, PMID:18003980, PMID:18063581, PMID:23418352
• GBF1 may participate in COPI-dependent recruitment of PNPLA2 to lipid droplets and contributes to maintaining mitochondrial morphology. Related references: PMID:19461073, PMID:22185782, PMID:25190516
• In neutrophils, it mediates GPCR-stimulated chemotaxis and superoxide production by recruiting and activating ARF1 at the leading edge, and facilitating assembly of the NADPH oxidase complex. Related references: PMID:22573891
• GBF1 is ubiquitously expressed and associated with Charcot-Marie-Tooth disease and neurodegeneration.

Experimental Guidance and Technical Tips

• Given the high molecular weight of GBF1 (206 kDa), consider using low-percentage SDS-PAGE gels (e.g., 6-8%) for optimal resolution in Western blotting.
• Validate the antibody in relevant human and mouse cell lines known to express GBF1; ubiquitous expression suggests most cell types are suitable.
• For immunofluorescence, optimize fixation and permeabilization conditions to preserve Golgi morphology and allow antibody access to this membrane-associated protein.
• The immunogen corresponds to the N-terminal amino acids 1-85, which may provide specificity across isoforms; verify in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supports diagnostic manufacturers and researchers with validated antibodies like this anti-GBF1 polyclonal, ideal for studies in Golgi biology and vesicular trafficking. Beyond individual antibodies, we provide optimized antibody pairs, bulk ancillary reagents, and sourcing services for rare targets, streamlining your development from discovery to clinic. Our integrated supply chain ensures consistent quality, helping you accelerate assay development for neurodegeneration and cell biology applications.