GATA1 Rabbit Polyclonal Antibody for WB - P15976

Core Product Specifications and Parameters

Biological Background: GATA1 Function and Localization

• GATA1, also known as GF-1, ERYF1, or NF-E1 DNA-binding protein, is a member of the GATA family of transcription factors.
• Serves as a general switch factor for erythroid development by binding to the consensus sequence 5'-[AT]GATA[AG]-3' in regulatory regions of erythroid-expressed genes. Related references: PMID:35030251
• Activates the transcription of key erythroid differentiation genes such as HBB, HBG1/2, ALAS2, and HMBS. Related references: PMID:24245781
• Localizes to the nucleus, consistent with its function as a transcriptional regulator.
• Exhibits tissue-specific expression in erythrocytes, highlighting its critical role in erythropoiesis.
• Contains two C2H2-type zinc finger domains essential for DNA binding and protein-protein interactions.
• Undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, modulating transcriptional activity.
• Mutations in GATA1 are linked to hematological disorders such as hereditary hemolytic anemia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 55-155 of human GATA1; verify that this sequence is conserved across your target species if cross-reactivity data is not available.
• For Western blotting, start with a typical polyclonal antibody dilution (e.g., 1:500–1:2000) and optimize based on your sample type and detection system.
• This antibody is validated for reactivity with mouse GATA1; confirm and titrate for any other species or applications not listed.
• Because GATA1 is a nuclear transcription factor, nuclear enrichment protocols or fractionation may enhance detection sensitivity in whole-cell lysates.
• Given its role as a master erythroid regulator, consider functional validation in erythroid cell lines or primary erythroblasts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner, providing diagnostic manufacturers and research institutions with access to a broad portfolio of validated antibodies and ancillary reagents. This anti-GATA1 polyclonal antibody supports research into erythroid development, hemoglobinopathies, and transcriptional control, complementing our offerings for hematology-focused assay development. Beyond catalog products, we leverage global supply networks to procure rare target reagents, optimized monoclonal/polyclonal antibodies, and bulk ancillary materials, helping you streamline your pipeline from concept to clinic.