GAMT Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14353

Core Product Specifications and Parameters

Biological Background: GAMT Function and Localization

• GAMT encodes a methyltransferase enzyme that converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor, a process essential for energy metabolism and nervous system development. Related references: PMID:24415674 PMID:26003046 PMID:26319512
• The GAMT protein is subject to acetylation and other post-translational modifications, which may regulate its activity.
• Alternative splicing of the GAMT gene gives rise to multiple transcript variants; mutations in GAMT are linked to cerebral creatine deficiency syndromes.
• GAMT is predominantly expressed in the liver, consistent with its key role in creatine biosynthesis.
• The canonical GAMT protein has a calculated molecular weight of about 26.3 kDa, consistent with the observed band at ~26 kDa in western blotting.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to full-length human GAMT (aa 1-236), suggesting broad epitope recognition. For western blot, a band at approximately 26 kDa is expected; include a positive control such as liver tissue lysate.
• For immunohistochemistry (IHC-P), consider using liver sections where GAMT is highly expressed; optimize antigen retrieval conditions as needed.
• The antibody has shown cross-reactivity with mouse and rat GAMT; validate performance in your specific experimental system.
• This polyclonal antibody may be suitable for ELISA and immunofluorescence applications; appropriate secondary detection and optimization are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibodies like anti-GAMT, to support research in creatine metabolism and neurodevelopment. Our platform provides efficient sourcing of polyclonal and monoclonal antibodies, bulk reagents, and ancillary products, streamlining your development from concept to clinic.