GALNS Rabbit pAb for WB - P34059

Core Product Specifications and Parameters

Biological Background: GALNS Function and Localization

• GALNS encodes N-acetylgalactosamine-6-sulfatase, a lysosomal hydrolase also known as chondroitinsulfatase, galactose-6-sulfate sulfatase (GalN6S), and GalNAc6S sulfatase. This enzyme removes sulfate groups from glycosaminoglycans (GAGs).
• The primary substrates of GALNS are keratan sulfate (KS) and chondroitin-6-sulfate (C6S), which are key components of cartilage and connective tissues. Deficiency leads to accumulation of these GAGs.
• GALNS is localized within the lysosome and requires calcium ions for catalytic activity, classified as a metal-binding sulfatase.
• Post-translational modifications include glycosylation and the presence of disulfide bonds essential for its tertiary structure. The protein is synthesized with a signal peptide that directs it to the lysosome.
• Mutations in the GALNS gene are associated with mucopolysaccharidosis IVA (Morquio A syndrome), an autosomal recessive lysosomal storage disorder characterized by skeletal dysplasia and dwarfism.
• The enzyme belongs to the sulfatase family and is involved in the stepwise degradation of GAGs in lysosomes, critical for normal cellular turnover of extracellular matrix components.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 27-307 of human GALNS (NP_000503.1), which should elicit antibodies recognizing the mature protein across human, mouse, and rat species due to cross-reactivity.
• For Western blotting, a band at approximately 58 kDa is expected; consider using lysosomal-enriched fractions or tissue extracts from cartilage or liver for optimal detection.
• ELISA protocols may be developed for quantifying GALNS levels in biological fluids or cell lysates; validate the assay with known standards and include appropriate controls.
• Given the lysosomal localization, ensure sample preparation includes steps to release the enzyme from lysosomes (e.g., freeze-thaw cycles, detergent lysis) for accurate detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for the IVD industry, offering a wide range of raw materials including validated antibodies, bulk ancillary reagents, and rare target products. For mucopolysaccharidosis and lysosomal storage disease research, we supply optimized antibodies like this GALNS Rabbit pAb, enabling reliable diagnostic development. Whether you need antibody pairs, recombinant proteins, or custom sourcing, CamelBio streamlines your path from concept to clinic.