Galectin 3/LGALS3 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - P17931

Core Product Specifications and Parameters

Biological Background: Galectin-3 Function and Localization

• Galectin-3 (also known as 35 kDa lectin, CBP35, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, and Mac-2 antigen) is a galactose-specific lectin encoded by the LGALS3 gene in humans.
• It binds IgE and mediates CSPG4-stimulated endothelial cell migration via interaction with alpha-3, beta-1 integrin. Together with DMBT1, it is required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
• In the nucleus, Galectin-3 functions as a pre-mRNA splicing factor, participating in mRNA processing and splicing.
• It plays a role in acute inflammatory responses, including neutrophil activation and adhesion, chemoattraction of monocytes/macrophages, opsonization of apoptotic neutrophils, and mast cell activation.
• Together with TRIM16, Galectin-3 coordinates the recognition of damaged endomembranes with mobilization of core autophagy regulators ATG16L1 and BECN1, linking innate immunity to autophagy.
• The protein localizes to the cytoplasm, nucleus, and is also secreted. Major expression is found in colonic epithelium, activated macrophages, and fetal membranes.
• Keywords associated with Galectin-3 include acetylation, phosphorylation, disulfide bond, immunity, lectin, spliceosome, and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–100 of human Galectin-3. This N-terminal region is distinct from the carbohydrate-recognition domain and may aid in detecting both full-length and cleaved forms.
• For Western blotting, the predicted molecular weight is approximately 26 kDa, but due to post-translational modifications and the protein’s propensity to form multimers, both monomeric and higher molecular weight bands may be observed; consider including reducing agents in sample buffers.
• In immunohistochemistry (IHC-P), note that Galectin-3 can be cytoplasmic, nuclear, or secreted; antigen retrieval methods may be required, and appropriate controls should be included to confirm staining specificity.
• The antibody cross-reacts with mouse and rat Galectin-3, making it suitable for translational studies across these species; however, validate expression levels in the target tissue before quantitative comparisons.
• For immunoprecipitation (IP) and ELISA, the monoclonal nature of the antibody provides consistent batch-to-batch performance; optimize antibody concentration empirically for each assay format.
• Always include appropriate positive and negative controls, such as known Galectin-3-expressing cell lines (e.g., macrophages, colonic epithelial cells) and knockout or knockdown models where possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive sourcing solutions for IVD raw materials, including validated monoclonal antibodies like this anti-Galectin-3 clone. Beyond individual antibodies, we offer optimized antibody pairs, bulk ancillary reagents, and logistical support for rare-target procurement, streamlining your workflow from concept to clinic. With deep expertise in inflammation and cancer biomarker development, our team assists in integrating Galectin-3 detection into your assay platforms efficiently.