GABBR1 Rabbit pAb - Q9UBS5

Core Product Specifications and Parameters

Biological Background: GABBR1 Function and Localization

• GABBR1 is a component of the heterodimeric GABA(B) receptor, forming a functional receptor with GABBR2; within this complex, GABBR1 is responsible for agonist binding, while GABBR2 couples to G proteins. Related references: PMID:15617512, PMID:18165688, PMID:22660477

• Upon GABA binding, the receptor triggers signaling through G proteins, inhibiting adenylate cyclase, stimulating phospholipase A2, activating potassium channels, and inactivating calcium channels, which modulates synaptic transmission and neuronal excitability. Related references: PMID:10075644, PMID:10773016, PMID:10906333, PMID:9844003

• Presynaptic GABBR1 inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, while postsynaptic receptors activate inwardly rectifying potassium channels to generate slow inhibitory postsynaptic potentials. Related references: PMID:10075644, PMID:22660477, PMID:9844003

• Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding, potentially explaining differential ligand affinity at central versus peripheral sites.

• GABBR1 is highly expressed in brain, with isoform 1A mainly in granular cell and molecular layer, isoform 1B mainly in Purkinje cells, and isoform 1E predominantly in peripheral tissues (kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus, mammary gland). Related references: PMID:9753614, PMID:9844003, PMID:9872744, PMID:10906333

• Subcellularly, GABBR1 resides at the cell membrane, postsynaptic membrane, dendrites, and can also be secreted.

• GABBR1 is a glycoprotein with a signal peptide, sushi domain, coiled-coil region, and seven-transmembrane helices, and undergoes alternative splicing to generate isoforms with distinct tissue distributions.

• The receptor is activated by (-)-baclofen and blocked by phaclofen, and it plays roles in hippocampal long-term potentiation, slow wave sleep, muscle relaxation, and antinociception. Related references: PMID:24305054, PMID:9844003, PMID:9872316

Experimental Guidance and Technical Tips

• The immunogen targets the central region (aa 200-460) of human GABBR1, which may help detect multiple isoforms; however, isoform specificity should be validated in the relevant sample system.

• For Western blotting (WB), consider using reducing conditions and a positive control such as human, mouse, or rat brain tissue lysate; expect a band near 108 kDa.

• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization may be required for intracellular epitopes; validate staining patterns in cells or tissue sections known to express GABBR1.

• ELISA can be used for antibody titration or screening; ensure appropriate coating buffer and blocking conditions are optimized for the assay.

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